海南师范大学学报（自然科学版）2024，Vol.37Issue(2)：203-211,9.DOI:10.12051/j.issn.1674-4942.2024.02.010
豆腐柴叶果胶提取工艺优化
Optimization of the Extraction Process of Pectin from Premna Microphylla Turcz Leaf
摘要
Abstract
The effects of different extraction assays,influencing factors and extraction process parameters on the quality of Premna microphylla Turcz leaf pectin were investigated,modelled to obtain an optimal pectin extraction method and pro-cess parameters for good quality.Five different methods were used and compared to determine the best extraction method by comparing the pectin yield,galacturonic acid content and esterification degree.The Plackett-Burman test was used to se-lect the key factors from ultrasound time,ultrasound power,ultrasound temperature,material-liquid ratio,microwave time and microwave temperature in the ultrasound-microwave tandem extraction of pectin,and the Box-Behnken response sur-face test was used to optimize the parameters of the extraction process,to achieve the optimal parameters and then verify them.The results showed that the most suitable method for Premna microphylla Turcz leaf pectin extraction was ultrasonic microwave tandem-assisted hydrochloric acid method,and the key factors affecting the extraction of Premna microphylla Turcz leaf pectin by ultrasonic microwave tandem-assisted hydrochloric acid method were microwave time,ultrasond tem-perature and microwave temperature.,The regression model of the extraction process parameters of Premna microphylla Turcz leaf pectin established with the yield of pectin,the content of galacturonic acid and the degree of esterification was significant(P<0.05).The optimal extraction process parameters were 1:20 of material-liquid ratio,10 min of ultrasound time,30℃of ultrasound temperature,600 W of ultrasound power,30 min of microwave time,and 88℃of microwave tem-perature,obtaining 15.68%of the pectin yield of Premna microphylla Turcz leaf,83.24%of the content of galacturonic ac-id,and 70.25%of the degree of esterification.The study could provide a reference for the development and utilization of the pectin resources of Premna microphylla Turcz leaf.关键词
豆腐柴叶果胶/超声微波辅助提取/工艺优化/响应面Key words
Premna microphylla Turcz leaf pectin/ultrasonic-microwave assisted extraction/process optimization/response surface分类
轻工纺织引用本文复制引用
田媛,陈月圆,赵武奇,贺刘成,王唯..豆腐柴叶果胶提取工艺优化[J].海南师范大学学报（自然科学版）,2024,37(2):203-211,9.基金项目
西安市农业技术研发项目(22NYYF050) （22NYYF050）
