海南师范大学学报（自然科学版）2024，Vol.37Issue(2)：218-223,6.DOI:10.12051/j.issn.1674-4942.2024.02.012
HPLC法同时测定植物饮料中7种绿原酸
Simultaneous Determination of Seven Chlorogenic Acids in Plant Vegetable Beverages by HPLC
摘要
Abstract
An HPLC method was established for the simultaneous determination of seven chlorogenic acids in plant bever-ages,by using C18 chromatographic column,elutioned with acetonitrile+0.1%formic acid+0.1%acetic acid.The detec-tion wavelength was 327 nm and the reference wavelength was 400 nm.The detection limit of seven chlorogenic acids was 0.25 mg/L,and the relationship was linear in the concentration range of 0.5～20 μg/mL.The recoveries of neochloro genic acid,chlorogenic acid,cryptochlorogenic acid,isochlorogenic acid B,1,5-dicaveoyl quinic acid,isochlorogenic acid A and isochlorogenic acid C were 99%～108%,97.9%～101%,96.9%～102%,96.2%～106%,95.1%～96.7%,94.9%～99.6%,96.3%～108%,respectively.The method was simple,fast,accurate in qualitative and quantitative,and could simultaneously determine seven kinds of chlorogenic acids in plant beverages.关键词
高效液相色谱法/植物饮料/7种绿原酸Key words
HPLC/vegetable beverages/seven chlorogenic acids分类
化学化工引用本文复制引用
黄柳霞,许雪儿,李莲..HPLC法同时测定植物饮料中7种绿原酸[J].海南师范大学学报（自然科学版）,2024,37(2):218-223,6.基金项目
广东省食品质量安全服务工程技术研究中心(粤科函产字[2020]141号) （粤科函产字[2020]141号）
