花生学报2024，Vol.53Issue(2)：55-62,8.DOI:10.14001/j.issn.1002-4093.2024.02.007
贝莱斯芽孢杆菌HYN-9的分离、鉴定及其对花生白绢病的生防作用
Isolation and Identification of Bacillus velezensis HYN-9 and Its Biocontrol Efficacy against Sclerotium rolfsii
摘要
Abstract
This study aimed to obtain bacterial strains with high antagonistic effects against Scle-rotium rolfsii.In total,18 bacterial strains were isolated from Scutellaria baicalensis residue by plate coating method.Among these strains,HYN-9 exhibited the most robust antagonistic activity against S.rolfsii,with an inhibition zone width of 10.92 mm.The strain HYN-9 was identified as Bacillus velezensis based on morphological,physiological and biochemical characteristics,and 16 S rDNA se-quencing.The culture filtrate of HYN-9 potently inhibited the hyphal growth of S.rolfsii with an inhibition rate of 70.01%,and also had a strong inhibitory effect on the formation and germination of sclerotia.In addi-tion,the volatile compounds produced by strain HYN-9 inhibited the growth of S.rolfsii with an antibacteri-al rate of 88.79% .Pot experiment showed that HYN-9 significantly reduced the incidence rate and index of infection to peanut southern blight with the efficacy of 73.23%,which was significantly higher compared with carbendazim medicament(47.62%,P<0.05).Meanwhile,HYN-9 improved the activity of plant defense enzymes such as phenylalaninase(PAL),polyphenol oxidase(PPO),and peroxidase(POD),and so that enhanced the systemic resistance of the peanut plant against diseases.This study demonstrated that the strain HYN-9 possesses strong antagonistic effect against S.rolfsii,and has potential to be developed as biocontrol agents for peanut southern blight.关键词
白绢病菌/菌株筛选/拮抗/黄芩药渣/贝莱斯芽孢杆菌Key words
Sclerotium rolfsii/screening of strains/antagonistic/Scutellaria baicalensis residue/Bacillus velezensis分类
农业科技引用本文复制引用
李亮亮,李磊,甄静,陈国参,杨文玲,雷高,王继雯..贝莱斯芽孢杆菌HYN-9的分离、鉴定及其对花生白绢病的生防作用[J].花生学报,2024,53(2):55-62,8.基金项目
河南省科技攻关项目(222102110309) （222102110309）
河南省科学院重大科研聚焦项目(210105002) （210105002）
河南省科技研发计划联合基金项目(225200810095) （225200810095）
