黄瓜FRK基因家族的鉴定及其表达分析OA北大核心CSTPCD

Fructokinase(FRK)is the main catalytic enzyme in the phosphorylation of fructose and plays a key role in the regulation of plant growth and development.In order to further study the FRK gene function of Cucumis sativus,the FRK gene family and its molecular characteristics were systematically identified and analyzed,and the gene expression patterns under abiotic stress were analyzed.The results showed that there were 12 FRK genes distributed on 7 chromosomes.The number of encoded amino acids was 331～588,and the molecular weight was 35.63～65.90 kD.The FRK gene family of cucumber was divided into 3 subfamilies by phylogenetic analysis,and the gene structure and motif in each subfamily were basically the same.The promoter sequences mainly contained cis-acting elements such as light response,low temperature response,drought response,hormone response,defense stress and plant growth and development.Tissue-specific expression analysis showed that the expression levels of some FRK genes in cucumber were low or not expressed in roots,stems,leaves,female flowers,and male flowers,indicated that different cucumber FRK genes had tissue-specific expression patterns.Abiotic stress expression analysis showed that CsaFRK6,CsaFRK8 and CsaFRK12 was significantly up-regulated under low temperature stress(P＜0.05).The expression of CsaFRK2,CsaFRK5 and CsaFRK7 was significantly up-regulated under salt stress(P＜0.05).CsaFRK1～CsaFRK9 were significantly up-regulated under drought stress(P＜0.05).The expression patterns of FRK genes in different tissues and organs were different in response to abiotic stress.This study provides a reference for elucidating the biological gene function of cucumber and its response to abiotic stress.