马尾松超氧化物歧化酶(SOD)基因家族鉴定及表达分析OA北大核心CSTPCD

Superoxide dismutase(SOD)is a key enzyme in the plant antioxidant system that protects plants from biotic and abiotic stresses by removing reactive oxygen species produced by living organisms.As a pioneer tree species with strong drought resistance,the study on the response mechanism of masson pine(Pinus massoniana)to high temperature and drought will help to understand the stress tolerance mechanism of plants.In this study,the transcriptome data of masson pine in drought,high temperature and high temperature-drought combined stress were mined,the PmSOD gene family was identified and bioinformatically analyzed,and the gene expression pattern of PmSOD gene family under high temperature,drought and high temperature-drought stress was detected.The results showed that 8 members of the PmSOD gene family were identified,including 4 Cu/Zn-SODs(CSDs),2 Fe-SODs(FSDs)and 2 Mn-SODs(MSDs).Family members of the same type had the same protein domain,similar subcellular localization,and conserved motifs.The gene expression analysis showed that 3 types of stresses induced specific expression of PmSOD gene family.The expression mode of PmSOD gene family under combined stress was different from that of single high temperature or drought stress.The expression of PmCSD1 and PmCSD4 were significantly up-regulated under various stress treatments,indicating that PmCSD1 and PmCSD4 might be key genes in the abiotic stress response of masson pine.This study provides basic data for in-depth discussion of the function and application of SOD in the growth and stress adaptation of masson pine.