农业生物技术学报2024，Vol.32Issue(7)：1543-1553,11.DOI:10.3969/j.issn.1674-7968.2024.07.007
马尾松超氧化物歧化酶(SOD)基因家族鉴定及表达分析
Identification and Expression Analysis of Superoxide Dismutase(SOD)Gene Family in Masson Pine(Pinus massoniana)
摘要
Abstract
Superoxide dismutase(SOD)is a key enzyme in the plant antioxidant system that protects plants from biotic and abiotic stresses by removing reactive oxygen species produced by living organisms.As a pioneer tree species with strong drought resistance,the study on the response mechanism of masson pine(Pinus massoniana)to high temperature and drought will help to understand the stress tolerance mechanism of plants.In this study,the transcriptome data of masson pine in drought,high temperature and high temperature-drought combined stress were mined,the PmSOD gene family was identified and bioinformatically analyzed,and the gene expression pattern of PmSOD gene family under high temperature,drought and high temperature-drought stress was detected.The results showed that 8 members of the PmSOD gene family were identified,including 4 Cu/Zn-SODs(CSDs),2 Fe-SODs(FSDs)and 2 Mn-SODs(MSDs).Family members of the same type had the same protein domain,similar subcellular localization,and conserved motifs.The gene expression analysis showed that 3 types of stresses induced specific expression of PmSOD gene family.The expression mode of PmSOD gene family under combined stress was different from that of single high temperature or drought stress.The expression of PmCSD1 and PmCSD4 were significantly up-regulated under various stress treatments,indicating that PmCSD1 and PmCSD4 might be key genes in the abiotic stress response of masson pine.This study provides basic data for in-depth discussion of the function and application of SOD in the growth and stress adaptation of masson pine.关键词
马尾松/超氧化物歧化酶(SOD)/高温/干旱/复合胁迫Key words
Masson pine/Superoxide dismutase(SOD)/High temperature/Drought/Combined stress分类
农业科技引用本文复制引用
李燕,李良良,丁贵杰..马尾松超氧化物歧化酶(SOD)基因家族鉴定及表达分析[J].农业生物技术学报,2024,32(7):1543-1553,11.基金项目
贵州省科技计划项目后补助计划项目(黔科合平台人才[2018]5261) （黔科合平台人才[2018]5261）
贵州省科技计划项目(黔科合基础-ZK[2022]一般274) （黔科合基础-ZK[2022]一般274）
