农业生物技术学报2024，Vol.32Issue(7)：1554-1563,10.DOI:10.3969/j.issn.1674-7968.2024.07.008
樱黄素通过Nrf2通路抑制脂多糖诱导的奶牛乳腺上皮细胞炎症反应和脂质过氧化
Prunetin Inhibits LPS-induced Inflammatory Response and Lipid Peroxidation in Bovine Mammary Epithelial Cells Through the Nrf2 Pathway
摘要
Abstract
Mastitis has a significant impact on the health and milk production of dairy cows(Bos taurus),resulting in reduced quality of dairy products and significant economic losses to the entire dairy industry.Prunetin(Pru)had biological activities such as anti allergic and anti-inflammatory properties.The aim of this study was to investigate the effects and mechanisms of prunetin on the inflammatory response and lipid peroxidation induced by lipopolysaccharide in bovine mammary epithelial cells(BMECs).The experiment was divided into control(CK)group,lipopolysaccharide(LPS)group,and lipopolysaccharide plus prunetin(LPS+Pru)groups at concentrations of 10,20,40,and 80 μmol/L,as well as a lipopolysaccharide plus ferrostatin-1(Fer-1)(LPS+Fer-1)group.The expression levels of inflammatory cytokines,nuclear factor κB(NF-κB),and nuclear factor erythroid 2-related factor 2/Kelch-like ECH-associated protein 1(Nrf2/Keap1)proteins were detected in each group.The results showed that LPS significantly upregulated the gene and protein levels of inflammatory cytokines such as tumor necrosis factor-α(TNF-α),interleukin-1(IL-1β),IL-6,and IL-8.LPS also increased the content of Fe2+,reactive oxygen species(ROS),and malondialdehyde(MDA).Prunetin downregulated the genes of TNF-α,IL-1β,IL-6,IL-8,and their protein expression,upregulated the gene of Nrf2 and its protein expression,inhibited the NF-κB signaling pathway,and reduced cell death.Additionally,prunetin decreased the levels of Fe2+,ROS,and MDA while increasing the gene expression levels of glutathione peroxidase 4(GPX4)and ferritin heavy polypeptide 1(FTH1).These findings suggested that LPS induced inflammatory responses and lipid peroxidation in BMECs.Prunetin inhibited lipid peroxidation by activating Nrf2 and inhibiting NF-κB,and reducing the inflammatory response of BMECs induced by LPS,thereby exerting cytoprotective effects.This study provides theoretical support for the reduction of LPS induced inflammatory response and oxidative damage in BMECs by prunetin,and provides new ideas for protecting breast health in B.taurus.关键词
奶牛乳腺上皮细胞(BMECs)/樱黄素/脂质过氧化/脂多糖(LPS)/核转录相关因子(Nrf2)/核因子κB(NF-κB)Key words
Bovine mammary epithelial cells(BMECs)/Prunetin/Lipid peroxidation/Lipopolysaccharide(LPS)/NF-E2-related factor 2(Nrf2)/Nuclear factor κB(NF-κB)分类
农业科技引用本文复制引用
姚端阳,兰政慧,王若冰,丰艳妮,付开强,李华涛,刘锡武,曹雷..樱黄素通过Nrf2通路抑制脂多糖诱导的奶牛乳腺上皮细胞炎症反应和脂质过氧化[J].农业生物技术学报,2024,32(7):1554-1563,10.基金项目
山东省高等学校青创科技支持计划(2021KJ102) （2021KJ102）
山东省自然科学基金(ZR2023MC027 （）
ZR2021MC111) （）
中央引导地方科技发展专项科技特派员行动计划(23-1-3-7-zyyd-nsh) （23-1-3-7-zyyd-nsh）
