农业生物技术学报2024，Vol.32Issue(7)：1564-1576,13.DOI:10.3969/j.issn.1674-7968.2024.07.009
江泉黑猪PLAG1基因多态性及其与生长性能的关联分析
PLAG1 Gene Polymorphism and Its Association Analysis with Growth Performance in Jiangquan Black Pigs(Sus scrofa)
摘要
Abstract
Plemorphic adenoma gene 1(PLAG1)plays an important role in regulating the growth and development of livestock and poultry.The purpose of this study was to investigate the relationship between SNP of PLAG1 gene and growth performance in Jiangquan black pig,Jiangquan black pigs weighing about 100 kg were used as research materials.PLAG1 gene mutations in 277 Jiangquan black pigs were detected by Mass ARRAY technology,and the association between SNP and growth traits was analyzed.The results showed that there were 3 SNP in the 5'regulatory region of PLAG1 genes,and 1 in the 3'regulatory region.The polymorphic information content(PIC)of g.75645278A>T and g.75693827A>G sites was 0.35,indicating moderate polymorphism(0.25＜PIC＜0.50).g.75686279G>A and g.75686320T>C sites showed low polymorphism(PIC＜0.25).The genetic analysis showed that only g.75686279G>A locus was in Hardy-Weinberg equilibrium,and the other loci were significantly deviated from Hardy-Weinberg equilibrium(P＜0.05).The results of linkage disequilibrium analysis and growth trait correlation showed that the g.75645278A>T and g.75693827A>G sites were completely linked,and had extremely significant effects on days to 100 kg(D100),chest circumference and abdominal circumference(P＜0.01).In g.75645278A>T sites,AA individuals with D100 were significantly higher than TT and TA individuals(P＜0.05).Chest and abdominal girth of TT and TA individuals were significantly higher than those of AA individuals(P＜0.05).Four dominant haplotypes(GCA,GTG,GTA,ATG)and 7 diplotypes were constructed with the loci g.75686279G>A,g.75686320T>C and g.75693827A>G.The 7 combined genotypes had significant effects on D100(P＜0.05),and had extremely significant effects on chest and abdominal circumference(P＜0.01).There was extremely significant interaction between haplotype GCA and ATG in chest and abdominal circumference(P＜0.01),and significant interaction between haplotype GCA and ATG in body height(P＜0.05).There was significant interaction between haplotype GTG and ATG on back height traits(P＜0.05).In summary,SNP of PLAG1 gene locus are significantly correlated with growth performance of Jiangquan black pigs,and g.75645278A>T and g.75693827A>G sites could be used as candidate molecular marker sites for growth performance of Jiangquan black pigs.This study provides theoretical support for the breeding of Jiangquan black pigs.关键词
江泉黑猪/多形性腺瘤基因1(PLAG1)/SNP/生长性能/单倍型分析/关联分析Key words
Jiangquan black pig/Pleomorphic adenoma gene 1(PLAG1)/SNP/Growth performance/Haplotype analysis/Association analysis分类
农业科技引用本文复制引用
马小燕,王文文,郑凤玲,刘汇鑫,许飞,陈伟,曾勇庆,唐辉..江泉黑猪PLAG1基因多态性及其与生长性能的关联分析[J].农业生物技术学报,2024,32(7):1564-1576,13.基金项目
"十四五"国家重点研发计划(2021YFD1301203) （2021YFD1301203）
山东省农业良种工程项目(2022LZGC003) （2022LZGC003）
