柯乐猪METTL23基因多态性与繁殖性能的关联性分析OA北大核心CSTPCD

Methyltransferase-like 23(METTL23)plays an important role in the development of early porcine(Sus scrofa)embryos,tissues and organs.To analyze the genetic effect of SNP locus of METTL23 gene on reproductive traits,the correlation between SNP locus and reproductive trait of Kele pigs was analyzed by SPSS 22.0 software,including live piglet number,birth litter weight,birth average weight,weaning piglet number,weaning litter weight and weaning weight in this study.The results showed that 3 SNPs mutation sites of g.4804958G>T(intron 1),g.4805082C>T(exon 2)and g.4806821A>G(exon 4)were found in Kele pig METTL23 gene,and there were 3 genotypes.GT,CC and AA were the dominant genotypes of the corresponding mutation sites,and G,C and A were the dominant alleles of the corresponding mutation sites.The polymorphic information content(PIC)was moderately polymorphic(0.25＜PIC＜0.50).Linkage disequilibrium analysis showed that there was no strong linkage disequilibrium effect among the 3 SNPs.Correlation analysis showed that g.4804958G>T locus had significant effects on live piglets,birth litter weight and weaned piglets(P＜0.05),and g.4806821A>G loci had significant effects on birth litter weight,average birth weight and weaning piglets(P＜0.05).Diplotype H4H4(GGCCGG)had significant effects on live piglets,birth litter weight,weaned piglets,weaning litter weight and average weaning weight(P＜0.01).G.4805082C>T and g.4806821A>G had significant effects on the reproductive performance of Kele pigs,and the diplotype H4H4(GGCCGG)had a very significant effect on the reproductive performance,so METTL23 could be used as an auxiliary marker for molecular breeding.This study provides a theoretical reference for pig genetics and breeding.