农业生物技术学报2024，Vol.32Issue(7)：1577-1586,10.DOI:10.3969/j.issn.1674-7968.2024.07.010
柯乐猪METTL23基因多态性与繁殖性能的关联性分析
Association Analysis of METTL23 Gene Polymorphisms with Reproductive Performance in Kele Pigs(Sus scrofa)
摘要
Abstract
Methyltransferase-like 23(METTL23)plays an important role in the development of early porcine(Sus scrofa)embryos,tissues and organs.To analyze the genetic effect of SNP locus of METTL23 gene on reproductive traits,the correlation between SNP locus and reproductive trait of Kele pigs was analyzed by SPSS 22.0 software,including live piglet number,birth litter weight,birth average weight,weaning piglet number,weaning litter weight and weaning weight in this study.The results showed that 3 SNPs mutation sites of g.4804958G>T(intron 1),g.4805082C>T(exon 2)and g.4806821A>G(exon 4)were found in Kele pig METTL23 gene,and there were 3 genotypes.GT,CC and AA were the dominant genotypes of the corresponding mutation sites,and G,C and A were the dominant alleles of the corresponding mutation sites.The polymorphic information content(PIC)was moderately polymorphic(0.25＜PIC＜0.50).Linkage disequilibrium analysis showed that there was no strong linkage disequilibrium effect among the 3 SNPs.Correlation analysis showed that g.4804958G>T locus had significant effects on live piglets,birth litter weight and weaned piglets(P＜0.05),and g.4806821A>G loci had significant effects on birth litter weight,average birth weight and weaning piglets(P＜0.05).Diplotype H4H4(GGCCGG)had significant effects on live piglets,birth litter weight,weaned piglets,weaning litter weight and average weaning weight(P＜0.01).G.4805082C>T and g.4806821A>G had significant effects on the reproductive performance of Kele pigs,and the diplotype H4H4(GGCCGG)had a very significant effect on the reproductive performance,so METTL23 could be used as an auxiliary marker for molecular breeding.This study provides a theoretical reference for pig genetics and breeding.关键词
甲基转移酶23(METTL23)/柯乐猪/多态性/关联性分析/繁殖性能Key words
Methyltransferase like 23(METTL23)/Kele pigs/Polymorphism/Association analysis/Reproductive performance分类
农业科技引用本文复制引用
向进,王春源,罗华伦,吴燕,杨酸,谭元成,张依裕..柯乐猪METTL23基因多态性与繁殖性能的关联性分析[J].农业生物技术学报,2024,32(7):1577-1586,10.基金项目
贵州省科技支撑计划(黔科合支撑[2021]一般147) （黔科合支撑[2021]一般147）
贵州省科技计划项目(黔科合平台人才[2021]5630号) （黔科合平台人才[2021]5630号）
贵州省生猪产业发展项目(黔财农[2021]157号 （）
黔财农[2022]183号) （）
贵州省种业发展项目(黔农计财[2022]10号) （黔农计财[2022]10号）
贵州省生猪产业技术体系(GZSZCYJSTX-03) （GZSZCYJSTX-03）
国家重点研发计划(2022YFD1100308-01) （2022YFD1100308-01）
