农业生物技术学报2024，Vol.32Issue(7)：1587-1595,9.DOI:10.3969/j.issn.1674-7968.2024.07.011
绵羊PRSS12基因多态性及其与生长性状的关联分析
Polymorphism of PRSS12 Gene and Its Association Analysis with Growth Traits in Sheep(Ovis aries)
摘要
Abstract
The identification of sheep(Ovis aries)molecular markers related to growth traits is of great significance to improve the economic benefits of sheep industry.Serine protease 12(PRSS12),also known as neurotrypsin,is specifically expressed in the central nervous system and is associated with a variety of neurological functions.In order to explore the polymorphism of PRSS12 gene and its correlation with the growth traits of Hu sheep and Ujumqin sheep,this study used gene chip technology to genotype 1008 Hu sheep and 336 Ujimqin sheep,and conducted association analysis with growth traits to identify molecular markers related to sheep growth and development.The results of genotyping showed that two SNP loci were detected in Ujimqin sheep population,in which rs402399470G>A presented low polymorphism,while rs418440631G>A showed moderate polymorphism.And only one SNP locus,namely rs418440631G>A,was detected in Hu sheep population,which showed low polymorphism.The results of association analysis with growth traits showed that rs402399470G>A locus was significantly correlated with 4-month-old body weight and 6-month-old chest width(P＜0.05),while rs418440631G>A locus was significantly correlated with 4-month-old chest circumference,tube circumference and 6-month-old chest circumference of Ujimqin sheep(P＜0.05).The rs418440631G>A locus was extremely significantly correlated with birth weight and 5-month chest circumference of Hu sheep(P＜0.01),and significantly correlated with 3-month tube circumference,5-month body length and chest width of Hu sheep(P＜0.05).The correlation coefficient between body weight and body size of Ujimqin sheep and Hu sheep was calculated by R language corrplot package,showing a significant positive correlation between weight and body size(P＜0.05),with the highest correlation coefficient between weight and chest circumference.There was a significant positive correlation between body weight and body size,and the correlation coefficient between weight and chest circumference was the highest.In conclusion,the rs402399470G>A and rs418440631G>A loci of PRSS12 gene could be used as potential molecular genetic markers for the growth traits of sheep.This study provides a reference basis for mutton sheep breeding and selection.关键词
丝氨酸蛋白酶12基因(PRSS12)/湖羊/乌珠穆沁羊/生长发育性状/关联分析Key words
Serine protease 12 gene(PRSS12)/Hu sheep/Ujimqin sheep/Growth and development trait/Association analysis分类
农业科技引用本文复制引用
熊金珂,鲍晶晶,毕亚珍,胡文萍,张莉..绵羊PRSS12基因多态性及其与生长性状的关联分析[J].农业生物技术学报,2024,32(7):1587-1595,9.基金项目
内蒙古自治区揭榜挂帅(2022JBGS0024) （2022JBGS0024）
新疆天池英才计划人才项目(新疆畜牧科学院) （新疆畜牧科学院）
国家重点研发计划(2021YFD1300902) （2021YFD1300902）
