农药学学报2024，Vol.26Issue(3)：540-548,9.DOI:10.16801/j.issn.1008-7303.2024.0028
己唑醇及其复配剂对河南省禾谷镰孢菌的抑制活性及对小麦赤霉病的室内防效
Inhibitory activity of hexaconazole and its mixtures against Fusarium graminearum in Henan Province and its indoor control efficacy against Fusarium head blight
摘要
Abstract
Fusarium graminearum is the dominant pathogen causing wheat Fusarium head blight(FHB)in Henan Province.To elucidate the inhibitory activity of hexaconazole against F.graminearum in Henan Province,this study determined the toxicity of hexaconazole on F.graminearum at different developmental stages and the inhibitory activity of hexaconazole against 205 F.graminearum strains collected from six regions in Henan Province was determined using the mycelial growth rate method.Finally,the coleoptiles of wheat inoculation were used to determine the indoor control efficacy of hexaconazole against FHB.The results indicated significant variations in inhibitory activity of hexaconazole against F.graminearum at different developmental stages,with EC50/(μg/mL)values ranking as follows:spore germination(22.5750)>sporulation quantity(2.1686)>sprout tube elongation(0.8922)>smycelium growth(0.3862).The EC50 value of hexaconazole against 205 strains of F.graminearum ranged from 0.0345 to 0.9439 μg/mL,with an average EC50 value of(0.3578±0.1928)μg/mL.The frequency distribution of sensitivity exhibited a continuous single-peak curve,suggesting the absence of subpopulations with reduced sensitivity to hexaconazole.The results of the combination toxicity between hexaconazole and carbendazim,fluoxazonil,phenamacril,prochloraz,and pydiflumetofen revealed various additive or synergistic effects.The synergy ratio(SR)ranged from 0.5042 to 3.7293,with the maximum value observed at a ratio ofm(hexaconazole):m(pydiflumetofen)=1:5(SR=3.7293),yielding an actual EC50 value of 0.0133 μg/mL.The results of indoor control efficacy showed that the combined fungicides exhibited significantly higher control efficacy compared to single fungicides,namely carbendazim,hexaconazole,and pydiflumetofen.The control efficacy of m(hexaconazole):m(pydiflumetofen)=1:5 on coleoptiles of wheat was 93.93%at a concentration of 9 μg/mL.In practical applications,hexaconazole should be combined or rotated with fluoxazonil or pydiflumetofen to prevent or delay the development of field resistance.This study provides crucial theoretical and data support for the rational use of hexaconazole and comprehensive control of FHB.关键词
己唑醇/杀菌剂复配/禾谷镰孢菌/抑制活性/小麦赤霉病/防治效果Key words
hexaconazole/fungicide mixture/Fusarium graminearum/inhibitory activity/Fusarium head blight/control efficacy分类
农业科技引用本文复制引用
李梦雨,高续恒,钱乐,姜佳,刘圣明..己唑醇及其复配剂对河南省禾谷镰孢菌的抑制活性及对小麦赤霉病的室内防效[J].农药学学报,2024,26(3):540-548,9.基金项目
河南省科技研发计划联合基金(232301420122) （232301420122）
洛阳市公益性行业科研专项(2302032A) （2302032A）
河南省自然科学基金(222300420145) （222300420145）
河南省科技攻关项目(242102110157). Supported by the Henan Provincial Science and Technology R&D Plan Joint Fund(No.232301420122) （242102110157）
Commonwealth Specialized Research Fund of Luoyang(No.2302032A) （No.2302032A）
Natural Science Foundation of Henan Province(No.222300420145) （No.222300420145）
Henan Province Science and Technology Research and Development Plan(No.242102110157). （No.242102110157）
