农药学学报2024，Vol.26Issue(3)：482-491,10.DOI:10.16801/j.issn.1008-7303.2024.0059
生防芽孢杆菌种子处理剂WS20的研制及对甘蓝枯萎病的防治效果
Development of biocontrol Bacillus seed treatment agent WS20 and control efficacy against cabbage Fusarium wilt
摘要
Abstract
Biocontrol Bacillus microbial preparations can effectively control plant diseases.In this study,the fermentation broth of Bacillus atrophaeus ZY1825 strain with biocontrol potential was used as the active ingredient of the coated seed treatment agent,and the causal agent of cabbage Fusarium wilt was used as the indicator pathogen.Single factor,orthogonal,plate confrontation test,and dilution plate counting methods were used to determine the antagonistic antibacterial effect and the content of viable bacteria.Through the screening of dispersant,film-forming agent,stabilizer,antifreeze,and other additives,the optimal formula of mixed additives was determined as follows:4.00%sodium lignosulfonate,0.25%carboxymethyl cellulose,1.00%bentonite,0.50%ethylene glycol,and 2.00%scarlet dye(mass fraction).The formulation of biocontrol Bacillus seed treatment agent WS20 was as follows:2:1 of the volume ratio for B.atrophaeus fermentation broth(9.28×109 cfu/mL)to mixed additives,and 1.45 min of film-forming time.The coating uniformity was 93.06%,and the shelf life was up to 150 d.The indexes of heat storage and low-temperature stability met the relevant national standards of seed treatment suspension agents and microbial preparation.The control effect on cabbage Fusarium wilt was 64.94%when the cabbage seeds were coated with WS20 at a mass ratio of 1:30.The research results can provide a reference for the preparation of biocontrol Bacillus seed treatment agents and the biological control of soil-borne diseases.关键词
种子处理剂/生防菌/萎缩芽孢杆菌/助剂筛选/甘蓝枯萎病/防治效果Key words
bio-seed coating agent/biocontrol bacteria/Bacillus atrophaeus/screening adjuvant/cabbage Fusariumwilt/control efficacy分类
化学化工引用本文复制引用
梁玉婷,任兴平,李惠霞,高有军,张海英,刘永刚..生防芽孢杆菌种子处理剂WS20的研制及对甘蓝枯萎病的防治效果[J].农药学学报,2024,26(3):482-491,10.基金项目
甘肃省重点人才项目(2023RCXM80) （2023RCXM80）
技术创新引导计划-东西协作项目(23CXNA0023) （23CXNA0023）
甘肃省农业科学院科研条件建设及成果转化项目(2021GAAS21) （2021GAAS21）
甘肃省中小企业创新基金(23CXGH0007). Supported by Gansu Provincial Key Talents Project Funding(No.2023RCXM80) （23CXGH0007）
Technology Innovation Guidance Program-East-West Collaboration Project(No.23CXNA0023) （No.23CXNA0023）
Gansu Academy of Agricultural Sciences Research Conditions Construction and Achievement Transformation Project(No.2021GAAS21) （No.2021GAAS21）
Gansu Province SME Innovation Fund(No.23CXGH0007). （No.23CXGH0007）
