农药学学报2024，Vol.26Issue(3)：517-525,9.DOI:10.16801/j.issn.1008-7303.2024.0019
响应面法优化诺尔斯链霉菌发酵条件以提高谷氏菌素产量
Optimization of fermentation conditions of Streptomyces noursei using response surface methodology to improve the gougerotin yield
摘要
Abstract
Streptomyces noursei was rich in secondary metabolites and had a broad-spectrum antifungal activity.A strain of S.noursei NK27,whose secondary metabolites had significant control effect on Botrytis cinerea was screened in our laboratory.In order to improve the ability of NK27 to produce gougerotin,single-factor tests,Plackett-Burman(PB)tests,the steepest climb tests and Box-Behnken tests were designed to optimize the fermentation conditions of NK27.From the five fermentation conditions,the three factors of inoculum age,inoculation amount and the initial pH showed the most significant influence on the yield of gougerotin.The fermentation conditions after the optimization of response surface were as follows:initial pH 7.6,inoculum age 32.6 h,inoculation amount 2.6%,rotation speed 220 r/min,fermentation time 144 h.The content of gougerotin was 3 times higher than that under the original fermentation condition.This study provided a theoretical basis for the industrial fermentation of S.noursei.关键词
响应面法/诺尔斯链霉菌/发酵条件/次级代谢产物/谷氏菌素Key words
response surface methodology/Streptomyces noursei/fermentation conditions/secondary metabolites/gougerotin分类
化学工程引用本文复制引用
宋巍,许春丽,施李鸣,陈丹,段小莉,李亚宁,葛蓓孛..响应面法优化诺尔斯链霉菌发酵条件以提高谷氏菌素产量[J].农药学学报,2024,26(3):517-525,9.基金项目
烟草行业烟草病虫害监测与综合治理重点实验室开放课题(KLTPMIMT2022-13) （KLTPMIMT2022-13）
中国烟草总公司重大科技项目(110202201019(LS-03)). Supported by Key Laboratory of Tobacco Pest Monitoring and Integrated Management in Tobacco Industry(No.KLTPMIMT2022-13)and Major Science and Technology Program of China Tobacco Corporation(No.110202201019(LS-03)). （110202201019(LS-03）
