农药学学报2024，Vol.26Issue(3)：619-624,6.DOI:10.16801/j.issn.1008-7303.2024.0026
丁子香酚对人参黑斑病菌的抑制活性及其作用机制研究
Antifungal effects and action mechanism of eugenol on Alternaria panax causing ginseng black spot
摘要
Abstract
Ginseng black spot caused by Alternaria panax is one of the most important fungal diseases,seriously affecting the yield and quality of Panax ginseng.The inhibitory effect of eugenol on A.panax was investigated using the mycelial growth rate and the spore germination methods.The effects of eugenol on relative conductivity,malondialdehyde content,and antioxidant enzyme activities of A.panax were evaluated.The results showed that eugenol significantly suppressed the growth and development of the pathogen.When the concentration of eugenol was 0.30 mg/mL,the inhibition rates on mycelial growth and spore germination were 90.14%and 94.40%,respectively.After 12 h treatment with eugenol,the relative conductivity and malondialdehyde content(MDA)of A.panaxincreased significantly compared with the control,which was 66.18%and 1.83 nmol/g,respectively.The activity of peroxidase(POD),catalase(CAT),and superoxide dismutase(SOD)peaked at 24 h,with 379.80,38.80,and 305.10 U/g respectively.Eugenol-induced programmed cell death exerts its antifungal function by damaging the integrity of the cell membrane,increasing lipid peroxidation,and promoting the accumulation of reactive oxygen species of A.panax.This study provided a reference for the rational application of eugenol in the green prevention and control of ginseng black spot,and increased the choice of fungicides.关键词
丁子香酚/人参黑斑病菌/抑菌活性/生理生化机理Key words
eugenol/Alternaria panax/antifungal activity/physiological and biochemical mechanism分类
农业科技引用本文复制引用
郭鸶怡,马榕,李思奇,李自博,朴静子,周如军..丁子香酚对人参黑斑病菌的抑制活性及其作用机制研究[J].农药学学报,2024,26(3):619-624,6.基金项目
中央本级重大增减支项目(2060302) （2060302）
沈阳农业大学科技供给计划项目(SNKJGJ202201). Supported by the Major Expenditure Increases or Decreases at the Central Level(No.2060302) （SNKJGJ202201）
Shenyang Agricultural University Science and Technology Supply Plan Project(No.SNKJGJ202201). （No.SNKJGJ202201）
