急性痛风性关节炎患者外周血黑色素瘤缺乏因子2信号通路相关细胞因子及焦孔素D表达变化观察OACSTPCD

Objective To observe the changes in the expression of absent in melanoma 2(AIM2),inflammasome signal pathway-related factors,double-stranded DNA(dsDNA),interleukin(IL)-1β,IL-18 and gasdermin D(GSDMD)in peripheral blood mononuclear cells(PBMCs)of patients with acute gouty arthritis,to analyze the relationship between AIM2,GSDMD in mononuclear cells and the condition of patients with acute gouty arthritis[WBC,PLT,ESR,CRP,IL-1β,IL-18,BMI,UA,BUN,serum creatinine(SCr),creatinine clearance rate(CCr),TG,TC],and to explore the role of AIM2 inflammasome signal pathway-mediated pyroptosis in the development and progression of acute gouty arthritis.Methods Thirty patients with acute gouty arthritis(observation group)and 30 healthy controls(control group)were in-cluded to compare the levels of AIM2,GSDMD in PBMCs,and IL-1β,IL-18,and dsDNA in serum.Spearman correla-tion analysis was used to investigate the relationships between AIM2 mRNA,GSDMD mRNA,and the related indicators of patients with acute gouty arthritis(IL-1β,IL-18,WBC,PLT,ESR,CRP,BMI,UA,BUN,SCr,CCr,TG,and TC).Results Compared with the control group,the expression levels of AIM2 and GSDMD mRNA in PBMCs of the observa-tion group were higher(both P<0.05),and the levels of serum dsDNA,IL-1β,IL-18 were higher(all P<0.01).AIM2 mRNA in PBMCs was positively correlated with WBC,ESR,CRP,BMI,TC,TG,and SCr(all P<0.05),and GSDMD mRNA was positively correlated with ESR,IL-1β,and SCr(all P<0.05).Conclusion The levels of AIM2,GSDMD in PBMCs,and dsDNA,IL-1β,and IL-18 in the serum of patients with acute gouty arthritis increase,indicating that AIM2 inflammasome-mediated pyroptosis may be involved in the development and progression of acute gouty arthritis.