山东医药2024，Vol.64Issue(15)：25-29,5.DOI:10.3969/j.issn.1002-266X.2024.15.005
急性痛风性关节炎患者外周血黑色素瘤缺乏因子2信号通路相关细胞因子及焦孔素D表达变化观察
Expression changes of AIM2 signal pathway-related cytokines and GSDMD in peripheral blood of patients with acute gouty arthritis
摘要
Abstract
Objective To observe the changes in the expression of absent in melanoma 2(AIM2),inflammasome signal pathway-related factors,double-stranded DNA(dsDNA),interleukin(IL)-1β,IL-18 and gasdermin D(GSDMD)in peripheral blood mononuclear cells(PBMCs)of patients with acute gouty arthritis,to analyze the relationship between AIM2,GSDMD in mononuclear cells and the condition of patients with acute gouty arthritis[WBC,PLT,ESR,CRP,IL-1β,IL-18,BMI,UA,BUN,serum creatinine(SCr),creatinine clearance rate(CCr),TG,TC],and to explore the role of AIM2 inflammasome signal pathway-mediated pyroptosis in the development and progression of acute gouty arthritis.Methods Thirty patients with acute gouty arthritis(observation group)and 30 healthy controls(control group)were in-cluded to compare the levels of AIM2,GSDMD in PBMCs,and IL-1β,IL-18,and dsDNA in serum.Spearman correla-tion analysis was used to investigate the relationships between AIM2 mRNA,GSDMD mRNA,and the related indicators of patients with acute gouty arthritis(IL-1β,IL-18,WBC,PLT,ESR,CRP,BMI,UA,BUN,SCr,CCr,TG,and TC).Results Compared with the control group,the expression levels of AIM2 and GSDMD mRNA in PBMCs of the observa-tion group were higher(both P<0.05),and the levels of serum dsDNA,IL-1β,IL-18 were higher(all P<0.01).AIM2 mRNA in PBMCs was positively correlated with WBC,ESR,CRP,BMI,TC,TG,and SCr(all P<0.05),and GSDMD mRNA was positively correlated with ESR,IL-1β,and SCr(all P<0.05).Conclusion The levels of AIM2,GSDMD in PBMCs,and dsDNA,IL-1β,and IL-18 in the serum of patients with acute gouty arthritis increase,indicating that AIM2 inflammasome-mediated pyroptosis may be involved in the development and progression of acute gouty arthritis.关键词
炎症小体/黑素瘤缺乏因子2/焦孔素D/双链DNA/白细胞介素1β/白细胞介素18/细胞焦亡/痛风性关节炎Key words
inflammasome/absent in melanoma 2/gasdermin D/double-stranded DNA/interleukin-1β/inter-leukin-18/pyroptosis/gouty arthritis分类
医药卫生引用本文复制引用
付笛语,田竞,初吉燕,郭琳,孙蕊,李萍..急性痛风性关节炎患者外周血黑色素瘤缺乏因子2信号通路相关细胞因子及焦孔素D表达变化观察[J].山东医药,2024,64(15):25-29,5.基金项目
辽宁省自然科学基金项目(2019-MS-350) （2019-MS-350）
联勤保障部队重点项目(19LBJ1003B). （19LBJ1003B）
