山东医药2024，Vol.64Issue(16)：6-9,14,5.DOI:10.3969/j.issn.1002-266X.2024.16.002
脑胶质瘤组织PLEKHA4表达变化及其影响因素分析
Expression changes of PLEKHA4 in glioma tissues and its influencing factors
摘要
Abstract
Objective To examine the changes in the expression levels of Pleckstrin homology domain family A member 4(PLEKHA4)in glioma tissues and its influencing factors.Methods Fifty-two cases of glioma tissues from sur-gical resection and 52 cases of their corresponding paraneoplastic tissues were selected,of which 21 cases were WHO grade Ⅰ-Ⅱ and 31 cases were grade Ⅲ-Ⅳ,and 23 cases were wild-type and 29 cases were mutant type of p53 typing.Im-munohistochemistry and protein immunoblotting were used to detect the expression level of PLEKHA4 in the paracancerous tissues and glioma tissues of patients with glioma.We observed the clinical characteristics and prognosis of glioma patients with different expression levels of PLEKHA4,and analyzed the factors affecting the expression level of PLEKHA4 in glio-ma tissues.Results PLEKHA4 was positively expressed in glioma tissues of 52 patients,and the sites of expression were mainly in the cytoplasm and cell membrane of glioma tissues;no positive expression of PLEKHA4 was seen in the paracancerous tissues.The relative expression levels of PLEKHA4 in the paracancerous tissues,grade Ⅰ-Ⅱ glioma,and grade Ⅲ-Ⅳ glioma were 0.419±0.101,0.968±0.061,and 1.366±0.096,respectively.The relative expression of PLEKHA4 in the glioma tissues was higher than that in paracancerous tissues,and the relative expression of PLEKHA4 was higher in glioma tissues of grade Ⅲ-Ⅳ than that in glioma tissues of grade Ⅰ-Ⅱ,respectively(both P<0.05).Signifi-cant differences were found in the WHO classification and p53 typing of patients with different PLEKHA4 expression levels in glioma tissues(both P<0.05).Logistic regression analysis showed that WHO classification and p53 typing of gliomas were the independent influencing factors for PLEKHA4 expression(both P<0.05).At 2-year postoperative follow-up,the survival rate of patients with high PLEKHA4 expression was 28.6%,the survival rate of patients with low PLEKHA4 expression was 70.8%,and statistically significant difference was found in the survival rate between them(P<0.05).Conclusion PLEKHA4 was highly expressed in glioma tissues,WHO typing and p53 typing of patients were the influ-encing factors of PLEKHA4 expression level in glioma tissues,and the PLEKHA4 expression level was related to clinical prognosis of patients.关键词
胶质瘤/Pleckstrin同源域A族成员4/胶质瘤WHO分型/胶质瘤p53分型/预后Key words
glioma/Pleckstrin homology domain-containing family A member 4/WHO classification/p53 typ-ing/prognosis引用本文复制引用
杜飞,李军鸿,吴秀杰,张健,张墨轩,郭守忠..脑胶质瘤组织PLEKHA4表达变化及其影响因素分析[J].山东医药,2024,64(16):6-9,14,5.基金项目
山东省医药卫生科技发展项目(202004090876). （202004090876）
