脑胶质瘤组织PLEKHA4表达变化及其影响因素分析OACSTPCD
Expression changes of PLEKHA4 in glioma tissues and its influencing factors
目的 探讨脑胶质瘤组织Pleckstrin同源域A族成员4(PLEKHA4)表达变化及其影响因素.方法 选取胶质瘤手术切除患者癌组织及相应癌旁组织标本52例,其中WHO分级Ⅰ～Ⅱ级21例、Ⅲ～Ⅳ级31例,p53分型野生型23例、突变型29例.采用免疫组化和免疫印迹两种方法检测脑胶质瘤患者癌旁组织、胶质瘤组织中的PLEKHA4表达,观察PLEKHA4不同表达水平胶质瘤患者的临床特点及临床预后情况,分析影响胶质瘤组织中PLEKHA4表达的相关因素.结果 52例患者胶质瘤组织PLEKHA4均为阳性表达,表达部位主要在胶质瘤组织细胞质和细胞膜中;癌旁组织未见PLEKHA4阳性表达.癌旁组织、Ⅰ～Ⅱ级胶质瘤、Ⅲ～Ⅳ级胶质瘤PLEKHA4相对表达量分别为0.419±0.101、0.968±0.061、1.366±0.096;PLEKHA4在胶质瘤组织中的相对表达量高于癌旁组织,Ⅲ～Ⅳ级胶质瘤组织PLEKHA4相对表达量高于Ⅰ～Ⅱ级胶质瘤组织(P均<0.05).胶质瘤组织不同PLEKHA4表达水平患者WHO分级、p53分型比较差异有统计学意义(P均<0.05).Logistic回归分析显示,胶质瘤WHO分级、p53分型是PLEKHA4表达的独立影响因素(P均<0.05).术后随访2年,PLEKHA4高表达患者生存率为28.6%,PLEKHA4 低表达患者生存率为 70.8%,二者生存率比较差异有统计学意义(P<0.05).结论 胶质瘤组织PLEKHA4高表达,患者WHO分型、p53分型是胶质瘤组织PLEKHA4表达的影响因素,PLEKHA4表达与患者临床预后相关.
Objective To examine the changes in the expression levels of Pleckstrin homology domain family A member 4(PLEKHA4)in glioma tissues and its influencing factors.Methods Fifty-two cases of glioma tissues from sur-gical resection and 52 cases of their corresponding paraneoplastic tissues were selected,of which 21 cases were WHO grade Ⅰ-Ⅱ and 31 cases were grade Ⅲ-Ⅳ,and 23 cases were wild-type and 29 cases were mutant type of p53 typing.Im-munohistochemistry and protein immunoblotting were used to detect the expression level of PLEKHA4 in the paracancerous tissues and glioma tissues of patients with glioma.We observed the clinical characteristics and prognosis of glioma patients with different expression levels of PLEKHA4,and analyzed the factors affecting the expression level of PLEKHA4 in glio-ma tissues.Results PLEKHA4 was positively expressed in glioma tissues of 52 patients,and the sites of expression were mainly in the cytoplasm and cell membrane of glioma tissues;no positive expression of PLEKHA4 was seen in the paracancerous tissues.The relative expression levels of PLEKHA4 in the paracancerous tissues,grade Ⅰ-Ⅱ glioma,and grade Ⅲ-Ⅳ glioma were 0.419±0.101,0.968±0.061,and 1.366±0.096,respectively.The relative expression of PLEKHA4 in the glioma tissues was higher than that in paracancerous tissues,and the relative expression of PLEKHA4 was higher in glioma tissues of grade Ⅲ-Ⅳ than that in glioma tissues of grade Ⅰ-Ⅱ,respectively(both P<0.05).Signifi-cant differences were found in the WHO classification and p53 typing of patients with different PLEKHA4 expression levels in glioma tissues(both P<0.05).Logistic regression analysis showed that WHO classification and p53 typing of gliomas were the independent influencing factors for PLEKHA4 expression(both P<0.05).At 2-year postoperative follow-up,the survival rate of patients with high PLEKHA4 expression was 28.6%,the survival rate of patients with low PLEKHA4 expression was 70.8%,and statistically significant difference was found in the survival rate between them(P<0.05).Conclusion PLEKHA4 was highly expressed in glioma tissues,WHO typing and p53 typing of patients were the influ-encing factors of PLEKHA4 expression level in glioma tissues,and the PLEKHA4 expression level was related to clinical prognosis of patients.
杜飞;李军鸿;吴秀杰;张健;张墨轩;郭守忠
山东第二医科大学临床医学院,山东潍坊 261053临沂市人民医院神经外科北京市神经外科研究所 首都医科大学附属北京天坛医院
胶质瘤Pleckstrin同源域A族成员4胶质瘤WHO分型胶质瘤p53分型预后
gliomaPleckstrin homology domain-containing family A member 4WHO classificationp53 typ-ingprognosis
《山东医药》 2024 (016)
6-9,14 / 5
山东省医药卫生科技发展项目(202004090876).
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