金钗石斛bHLH转录因子家族全基因组鉴定及表达分析OA北大核心CSTPCD

[Objective]bHLH transcription factors exist widely in plants and are one of the important transcription factor families that regulate plant growth and development and respond to stress.The DnbHLHs transcription factor family was identified from the whole genome of Dendrobium nobile and its expression characteristics were analyzed,which laid the foundation for further study on the biological function of DnbHLHs transcription factor.[Method]The bHLH transcription factor family members were identified from the whole genome of D.nobile by bioinformatics method,and their physical and chemical characteristics,phylogenetic relationship,gene structure,chromosome localization and promoter cis-acting elements were analyzed.Transcriptome data and RT-qPCR were used to analyze the tissue expression patterns of DnbHLHs transcription factors and their expression patterns under different abiotic stresses.[Result]A total of 137 bHLH transcription factors were identified in D.nobile.The amino acid number of DnbHLHs protein was 85-1 260,the molecular weight was 9 855.23-140 024.19 Da,the isoelectric point was 4.52-10.66,the instability coefficient was 31.94-87.42,the fat solubility index was 56.66-106.06,and the hydrophilic index was-0.815-0.185.DnbHLHs were distributed on 19 chromosomes of D.nobile,all of which had a bHLH domain and were homologous to the bHLH of Arabidopsis thaliana,and there are collinear relationships between groups and species.Analysis of gene expression patterns showed that DnbHLHs family members had tissue specificity,and DnbHLH26 and DnbHLH33 were responsive to various stresses.[Conclusion]A total of 137 DnbHLHs transcription factor family members are identified from the whole genome of D.nobile.The physicochemical properties and conserved mods of the family members showe specificity and diversity.There are tissue expression differences among different DnbHLHs transcription factors,and DnbHLH26 is mainly induced by high salt and high temperature stress.DnbHLH33 is mainly induced by high salt,high temperature and low temperature stress.