壳聚糖-蒙脱土插层复合物对热应激雄性小鼠生殖功能的影响OA北大核心CSTPCD

The protective effects of chitosan-montmorillonite intercalation complex(CS-MMT)on the clinical symptoms,testicular morphology,and sperm quality of heat-stressed male mice were studied.Thirty 6-week-old male ICR mice were randomly divided into control group(CON group),heat stress group(HS group),low-dose CS-MMT group(L group,50 mg/kg CS-MMT),middle-dose CS-MMT group(M group,100 mg/kg CS-MMT),and high-dose CS-MMT group(H group,200 mg/kg CS-MMT).The results showed that compared with CON group,the testicular tissue damage was obvious in HS group,sperm density was significantly decreased(P＜0.05),sperm viability and acrosome integrity were extremely decreased(P＜0.01),and the sperm malformation and acrosome malformation rates were extremely increased(P＜0.0l).Compared with HS group,the testicular tissue morphology and structure of mice in CS-MMT groups were significantly improved,the sperm density was increased,the sperm viability and acrosome integrity were extremely increased(P＜0.01),and the sperm malformation and acrosome malformation rates were extremely decreased(P＜0.01)in a dose-dependent manner.The study indicates that CS-MMT can alleviate the reproductive function damage induced by heat stress in male mice,and and the dosage of 200 mg/kg is better.