比利时杜鹃花类黄酮3'-羟化酶(F3'H)基因克隆及功能分析OA北大核心CSTPCD
Cloning and Functional Analysis of Flavonoid 3'-hydroxylase(F3'H)Gene in Rhododendron hybridum Hort
[目的]类黄酮 3'-羟化酶(flavanone 3'-hydroxylase,F3'H)是植物花青素合成过程中的关键酶,探究比利时杜鹃花(Rhododendron hybridum Hort)F3'H基因的功能及表达特性.[方法]以比利时杜鹃花不同发育时期的花瓣及盛花期的根、茎、叶为实验材料,从比利时杜鹃花转录本数据库中筛选类黄酮生物合成通路关键酶类黄酮 3'-羟化酶基因序列信息并进行生物信息学分析,利用反转录(RT-PCR)技术克隆RhF3'…查看全部>>
[Objective]The flavonoid 3'-hydroxylase(F3'H)is a key enzyme in the synthesis of anthocyanins in plants,and the function and expression characteristics of F3'H gene in Rhododendron hybridum Hort were investigated.[Method]The petals and roots,stems and leaves of R.hybridum Hort at different developmental stages were used as experimental materials.The flavonoid 3'-hydroxylase gene sequence information of flavonoid biosynthesis pathway was screened from the R.h…查看全部>>
吴泽航;杨中义;鄢毅铖;贾永红;吴月燕;谢晓鸿
浙江万里学院生物与环境学院,宁波 315100浙江万里学院生物与环境学院,宁波 315100浙江万里学院生物与环境学院,宁波 315100浙江万里学院生物与环境学院,宁波 315100浙江万里学院生物与环境学院,宁波 315100浙江万里学院设计艺术与建筑学院,宁波 315100
比利时杜鹃花类黄酮3'-羟化酶(F3'H)花青素亚细胞定位瞬时表达
Rhododendron hybridum Hortflavanone 3'-hydroxylaseanthocyanidinsubcellular localizationinstantaneous expression
《生物技术通报》 2024 (6)
251-259,9
宁波市科技创新 2025 现代种业重大专项(2021Z005),浙江省重点研发计划(2021C02053),省一流学科学生创新项目(CX2022023)
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