日用化学工业（中英文）2024，Vol.54Issue(6)：648-655,8.DOI:10.3969/j.issn.2097-2806.2024.06.004
全氟辛酸对成骨细胞骨形成和破骨细胞骨吸收作用研究
Effects of PFOA on osteoblastic bone formation and osteoclastic bone resorption
摘要
Abstract
Perfluorooctanoic acid(PFOA)exposure level in human is negatively correlated with bone density according to the epidemiological research.Our study is aim to investigate the effects and mechanisms of PFOA on osteoblastic bone formation and osteoclastic bone resorption.Primary osteoblasts were isolated from the calvaria of newborn rats,and the cell proliferation,alkaline phosphatase(ALP)activity,and bone nodule formation were detected after 1,10,and 100 μmol/L PFOA treateatment for 24 h.The osteoclasts model were differentiation from bone marrow monocyte,which induced by Macrophage colony-stimulating factor(M-CSF)and Nuclear factorκB receptor-activating factor ligand(RANKL),and the effects on cell viability,anti-tartaric acid phosphatase(TRAP)activity,and bone resorption pit formation were investigated.The expression of OPG and RANKL proteins in osteoblasts were conducted by Western blot.The results show that PFOA at the concentration of 1 μmol/L and 10 μmol/L can promote osteoblast proliferation,but has no effect on ALP activity and bone nodule formation.100 μmol/L PFOA can significantly inhibit cell proliferation,ALP activity,and bone nodule formation,as well as the proportion of OPG/RANKL protein expression.PFOA has no effect on osteoclast viability,and 10 μmol/L and 100 μmol/L PFOA can significantly inhibit the TRAP activity and the area of bone resorption pits.The conclusion can be draw that high concentration of PFOA can inhibit the bone formation of osteoblasts by suppressing WNT pathway and promote osteoclast bone resorption by inhibiting the OPG/RANKL/RANK pathway.关键词
全氟辛酸/骨丢失/成骨细胞/破骨细胞/OPG/RANKL/RANK通路Key words
perfluorooctanoic acid/bone loss/osteoblast/osteoclast/OPG/RANKL/RANK pathway分类
化学化工引用本文复制引用
薛黎明,林元杰,金玉娥,徐佳乐,卢大胜,汪国权..全氟辛酸对成骨细胞骨形成和破骨细胞骨吸收作用研究[J].日用化学工业（中英文）,2024,54(6):648-655,8.基金项目
上海市自然科学基金面上项目(21ZR1454600,22ZR1453400) （21ZR1454600,22ZR1453400）
上海市"医苑新星"青年医学人才-公共卫生领导者项目(沪卫人事[2021]99号) （沪卫人事[2021]99号）
上海市卫生健康委员会面上项目(202040208) （202040208）
上海市社会发展科技攻关项目(21DZ1202100) （21DZ1202100）
上海市加强公共卫生体系建设三年行动计划(2023-2025年)重点学科(GWVI-11.1-41) （2023-2025年）
