羟基积雪草苷通过激活Nrf2-HO-1通路发挥抗衰老及皮肤修复作用OA北大核心CSTPCD

The study aimed to investigate the anti-aging and skin repair effects of madecassoside(MA)on D-galactose-induced skin aging mice.Mice were divided into 6 groups(n=12):normal group(N),model group(M),low,medium,high-dose MA groups(L-MA,M-MA,H-MA)and high-dose MA+Nrf2 inhibitor ML385 group(H-MA+ML385).The body weight,skin water content,antioxidant indicators(SOD,CAT,GSH-Px and MDA),HYP and HA levels in skin tissue of mice in each group were detected.Skin histology was evaluated by HE staining.The transcript levels of MMP-1,MMP-3,Nrf2,Keap1 and HO-1 in skin tissues were detected by RT-qPCR.The protein expression levels of Nrf2,Keap1 and HO-1 in skin tissue were detected by Western blotting.The results show that compared with the M group,the skin water content of mice in L-MA group,M-MA group and H-MA group increases,the thickness of the epidermis and dermis increases,the skin structure is significantly improved,the levels of SOD,CAT and GSH-Px increase,the level of MDA decreases,the level of HYP and HA increases,the relative expression of MMP-1 and MMP-3 mRNA decreases,the relative expression of Nrf2 and HO-1 mRNA and protein increases,and the relative expression of Keap1 mRNA and protein decrease(P＜0.05).ML385 partially weakens the activation effect of MA on the Nrf2-HO-1 pathway,and weakens the anti-aging and skin repair effects of MA(P＜0.05).This study shows that MA has good anti-aging and skin repair effects,and its mechanism is related to the activation of Nrf2-HO-1 pathway.