山西农业大学学报（自然科学版）2024，Vol.44Issue(3)：1-13,13.DOI:10.13842/j.cnki.issn1671-8151.202312035
陆地棉YTH基因家族的鉴定与表达特征分析
Identification and expression analysis of YTH gene family in cotton(Gossypium hirsutum L.)
摘要
Abstract
[Objective]The YT521-B homology(YTH)family proteins can recognize and bind N6-methyl-adenosine(m6A),regulating the mRNA metabolism process,playing an important role in plant growth,de-velopment,and stress response.Exploring the tissue expression patterns and response of the cotton YTH gene family,as well as their response to exogenous hormones and abiotic stresses treatments,can provide a theoretical basis for a deeper understanding of the function of cotton GhYTHs genes.[Methods]In this study,the genome-wide identification of YTH genes in Gossypium hirsutum was performed and the basic characteris-tics were analyzed using bioinformatics methods.The expression patterns of GhYTH genes under abiotic stress and exogenous hormone treatment were analyzed using online RNA-Seq data and/or quantitative real-time PCR.[Results]The results showed that a total of 26 GhYTH genes were identified from G.hirsutum,unevenly distributed on 16 chromosomes,including 22 members of the YTH-DF subfamily and four mem-bers of the YTH-DC subfamily.GhYTHs exhibited similar motifs and gene structures.Tandem duplication and fragment duplication were the main driving forces for expansion of YTH gene family in G.hirsutum.The Ka/Ks ratio of the collinear genes between G.hirsutum and Arabidopsis were less than 0.5,indicating strong purifying selection during the evolution process of this gene family.Transcriptome results showed most GhYTHs showed tissue-specific expression patterns,with GhYTH2/16 showing high expression in various tissues.Abiotic stress significantly increased the expression of GhYTHs,especially GhYTH9/12/14,which was consistent with the qPCR results.Additionally,exogenous treatments with gibberellin,abscisic acid and methyl jasmonate led to a significant increase in the expression of GhYTH10/12/14.[Conclusion]Through whole-genome analysis of G.hirsutum,five important candidate genes,including GhYTH2/9/10/12/14,were identified.They may play important roles in cotton growth,development,and stress resistance,provid-ing important theoretical basis for further research on the role of YTH-mediated m6A modification in cotton.关键词
陆地棉/YTH基因/激素/胁迫/表达Key words
Gossypium hirsutum/YTH gene/Hormone/Stress/Expression分类
农业科技引用本文复制引用
钱娇,方佳琪,李迎迎,王丽..陆地棉YTH基因家族的鉴定与表达特征分析[J].山西农业大学学报（自然科学版）,2024,44(3):1-13,13.基金项目
国家自然科学基金项目(31601241) （31601241）
棉花生物育种与综合利用全国重点实验室开放课题基金(CB2023A07) （CB2023A07）
