山西农业大学学报（自然科学版）2024，Vol.44Issue(3)：66-74,9.DOI:10.13842/j.cnki.issn1671-8151.202311035
降解膜对高粱根际土壤微生物群落结构的影响
Effects of biodegradable films on rhizosphere soil microbial community structure in sorghum
摘要
Abstract
[Objective]The film is an important covering material for increasing crop yield and income in agricultural production.Biodegradable ground film,as a new type of soil covering material,can decompose into small molecules,eventually converting into carbon dioxide,water,and organic matter,playing an irreplaceable role in improving soil.This study aimed to clarify the influence of the biodegradable ground film on the characteristics of microbial community structure in farmland soil.[Methods]Three different degradable films were set:4-month degradation(4J),6-month degradation(6J),and common black film(PT),with non-filming field as the control(CK).The variation of soil bacterial community structure were analyzed using high-throughput sequencing technology,and the changes in microbial community and its effects on microbial ecological environment under the coverage of biodegradable films in farmland soil were explored.[Results]Compared with the non-filming treatment,the contents of total nitrogen(except for the PT treatment),available phosphorus,available potassium,and organic matter in the soil were all increased after filming.Alphaproteobacteria,Actinobacteria,Acidobacteria_Gp1 and Gammmaproteobacte-ria were the common dominant bacterial groups in the rhizosphere soil of dual-season sorghum.Under the filming treatments of the first season,the relative abundance of Actinobacteria,Acidobacteria_Gp1 and Acidobacteria_Gp2 in the soil was higher than that in the non-filming soil;Under the filming treatment of the second season,the relative abundance of Deltaproteobacte-ria in the soil was significantly higher than that in the non-filming soil,and the number of Operational Taxonomic Unit(OUT)of soil bacteria was more than 2000,showing higher microbial diversity.Through KEGG metabolic pathway analysis,soil car-bohydrate metabolism,amino acid metabolism,metabolism of cofactors and vitamins,and metabolism of terpenoids and polyketides were higher under filming treatment of the second season than that of the first season,among which the 6J treat-ment was optimal.[Conclusion]In summary,a 6-month biodegradable film(6J treatment)can effectively improve soil physico-chemical properties,enhance biodegradation of soil rhizosphere bacterial communities,and increase microbial diversity and rich-ness.关键词
生物降解膜/根际土壤/微生物群落/高通量测序Key words
Biodegradable film/Rhizosphere soil/Microbial community/High-throughput sequencing分类
农业科技引用本文复制引用
刘玉兵,曹春信,黄洪明,卢华兵,吕学高,刘新华..降解膜对高粱根际土壤微生物群落结构的影响[J].山西农业大学学报（自然科学版）,2024,44(3):66-74,9.基金项目
中国农业科学院金华市院地合作项目(YDHZ2020KY04) （YDHZ2020KY04）
浙江省农业重大技术协同推广计划项目(2023ZDXT03-5) （2023ZDXT03-5）
