生物技术通报2024，Vol.40Issue(6)：161-171,11.DOI:10.13560/j.cnki.biotech.bull.1985.2023-1153
甘蓝型油菜REVEILLE家族鉴定及诱导表达分析
Identification and Induced-expression Analysis of REVEILLE Family in Brassica napus L.
摘要
Abstract
[Objective]REVEILLE family genes have important biological functions,but there are few studies on this family gene in Brassica napus L.This project was conducted to further understand the function of RVE family genes in B.napus and their relationship with abiotic stress.[Method]Bioinformatics methods were used to identify33,16 and 16 RVE genes in B.napus,B.rapa and B.oleracea,and to analyze their phylogenetic development,chromosome localization,gene structure,physical and chemical properties,and promoter cis-acting elements.[Result]All RVE proteins were classified into two subfamilies.The 33 BnaRVE were located on 18 chromosomes of the A sub-genome and 15 chromosomes of the C sub-genome.Most members belonged to relatively stable hydrophobic proteins.Most of the Ka/Ks values were<1,indicating that the family was subjected to strong purification selection.The gene structure varied greatly,and the number of introns ranged from 4 to 11.Collinearity analysis showed that there were large number of homologous genes in B.napus and Arabidopsis,B.rapa and B.oleracea.Most RVE family members of B.napus expressed RVE in the cotyledon and leaves,and the expression was the highest.BnaRVE promoter contained a large number of cis-acting elements related to hormone and biological stress.Through quantitative PCR analysis,the expressions of 4 BnaRVE genes were significantly upregulated under ABA and MeJA induction as well as low temperature stress.[Conclusion]Thirty-three members of the BnaRVE family were identified in the genome of B.napus.Different genes showed different expression patterns in different developmental stages and different tissues.Different genes have different expression patterns in response to various stresses.RVE family members have positive response function to ABA,MeJA and cold stress.关键词
甘蓝型油菜/RVE基因家族/生物信息学分析/逆境/植物激素/表达分析/表达谱Key words
Brassica napus L./RVE genes family/bioinformatics analysis/adverse stress/phytohormone/expression analysis/expression profile引用本文复制引用
刘蓉,田闵玉,李光泽,谭成方,阮颖,刘春林..甘蓝型油菜REVEILLE家族鉴定及诱导表达分析[J].生物技术通报,2024,40(6):161-171,11.基金项目
国家自然基金委联合重点项目(U20A2028),湖南省教育厅项目(20K070) （U20A2028）
