RPRD1B对三阴性乳腺癌细胞生物学功能及上皮间质转化的影响OACSTPCD

Objective To investigate the effects of regulatory nuclear pre-mRNA domain containing 1B(RPRD1B)on the prolifera-tion,apoptosis,invasion,migration and epithelial-mesenchymal transition(EMT)of triple-negative breast cancer(TNBC).Methods The expression level of RPRD1B in TNBC tissues and adjacent non-tumor tissues was detected by immunohistochemistry.The expression level of RPRD1B in TNBC cell lines MDA-MB-231 and HCC-1937 was detected by Western blot.The TNBC cell lines MDA-MB-231 and HCC-1937 were respectively transfected with sh-RPRD1B lentivirus(sh-RPRD1B group)and sh-NC lentivirus(sh-NC group).The downregulation efficiency was detected.CCK-8 assay was used to detect the cell proliferation.Flow cytometry was used to detect the cell apoptosis.Transwell assay was used to detect the cell invasion.The wound healing assay was used to detect the cell migration.Western blot and qRT-PCR were used to detect the expressions of EMT-related proteins(E-cadherin and N-cadherin)in TNBC cells.Results RPRD1B was expressed in TNBC tissues and in human TNBC cell lines,MDA-MB-231 and HCC-1937.CCK-8 analysis showed that the proliferation ability in sh-RPRD1B group was significantly decreased compared with sh-NC group(P＜0.01).Flow cytometry analysis showed that the apoptosis rate in sh-RPRD1B group was significantly increased compared with sh-NC group(P＜0.01).Transwell analysis showed that the invasion ability in sh-RPRD1B group was significantly reduced compared with sh-NC group(P＜0.01).The wound healing analysis showed that the migration ability in sh-RPRD1B group was significantly decreased com-pared with sh-NC group(P＜0.01).The expression level of EMT marker E-cadherin was elevated while the expression of N-cadherin was reduced in sh-RPRD1B group compared with sh-NC group.Conclusion Knockdown of RPRD1B could increase the apoptosis of TNBC cells and reduce the proliferation,which may be related to the epithelial mesenchymal transformation process.