枸橼酸莫沙必利对大鼠脑卒中急性期胃肠功能障碍的影响及其机制OACSTPCD

Objective To explore the effect of Mosapride Citrate on gastrointestinal dysfunction in acute stage of stroke in rats and investigate its relationship with SCF/C-kit pathway.Methods SD rats were randomly divided into five groups:normal group,stroke groups(cerebral hemorrhage group,cerebral infarction group),and mosapride groups(cerebral hemorrhage+mosapride group,cerebral infarction+mosapride group).The hemorrhage model was induced by autologous blood injection method,and the infarct model was induced by suture occlusion method.After successful modeling,the rats in normal group and stroke group were given physiological saline by gavage,while the rats in mosapride group were given mosapride(0.27 mg/d)by gavage twice a day.The rats were eutha-nized after seven days of administration.Hematoxylin eosin(HE)was used to observe pathological changes in rat gastrointestinal tissue,RT-PCR and Western blot were used to detect the expression levels of SCF,C-kit protein and mRNA.Results The gastroin-testinal cells in normal group were clear and intact.In stroke group,the gastrointestinal tissue was disordered and the cell infiltration was serious.The degree of gastric tissue degeneration in moxapride group was less than that in stroke group.Compared with normal group,the pathological score was significantly increased in stroke group(P＜0.05).Compared with stroke group,the pathological change scores were significantly decreased in moxapride group(P＜0.05).Compared with normal group,SCF and C-kit protein and mRNA expressions in stroke group were significantly decreased(P＜0.05).Compared with cerebral infarction group,the expression level of gastrointestinal SCF protein was significantly increased in cerebral hemorrhage group(P＜0.05),the expression of C-kit protein in ileum was significantly decreased(P＜0.05),and the expressions of SCF and C-kit mRNA in gastrointestinal tissue were sig-nificantly increased(P＜0.05).Compared with stroke group,the expressions of SCF and C-kit protein and mRNA in gastrointestinal tissue were significantly increased in moxapride group(P＜0.05).The expression of SCF protein in gastrointestinal tissue of rats in cerebral infarction+moxapride group was significantly higher than that in cerebral hemorrhage+moxapride group(P＜0.05),and the expression of C-kit protein was similar in the two groups(P＞0.05).Conclusion Mosapride can activate the SCF/C-kit pathway by upregulating the expression of SCF and C-kit,thereby reducing the occurrence of gastrointestinal disorders in acute stroke.