神经节苷脂对初生大鼠高胆红素血症脑组织AIF表达的调节作用OACSTPCD

Objective To investigate the effect of brain-derived hyperglycoside ganglioside(GM1)on the expression of apoptosis-inducing factor(AIF)in the hippocampal CA1 region of neonatal rats with hyperbilirubinemia.Methods A total of 120 SD rats aged 7 days were randomly divided into control group,model group and GM1 group.The hyperbilirubinemia model was established by intraperitoneal injection of 100 pg/g bilirubin solution.The rats in control group were injected intraperitoneally with same amount of 0.9％NaCl.The rats in GM1 group were intraperitoneally injected with GM1 10 μg/g once immediately after the establishment of hyperbilirubinemia model,and then intraperitoneally injected with GM1 once a day at the same dose before execution.The behaviors of rats in each group were observed at 6,24,48,72,96 h after modeling,and then the rats were sacrificed to collect brain tissue.Immunohistochemical staining and TUNEL staining were used to detect AIF protein expression and the neuronal apoptosis in the hippocampal CA1 region of brain tissue at different time points.Results In control group,there was no obvious AIF expression and apoptotic cells at each time point.In model group,AIF expression and apoptotic cells appeared in the hippocampus at 6 h,and gradually increased and peaked at 96 h.Compared with model group,AIF expression and the apoptosis in GM1 group were significantly decreased at each time point(P＜0.05).AIF expression and the apoptosis in GM1 group were still higher than those in control group at each time point(P＜0.05).Conclusion Ganglioside(GM 1)may play a protective role in neonatal rats with hyperbilirubinemia by inhibiting AIF expression and apoptosis in the hippocampal CA1 region of brain tissue.