塔里木大学学报2024，Vol.36Issue(2)：19-29,11.DOI:10.3969/j.issn.1009-0568.2024.02.003
不同临床型奶牛生殖道乳酸菌的筛选鉴定及菌群多样性分析
Screening,identification and diversity analysis of lactic acid bacteria in reproductive tract of dairy cows with different clinical types
摘要
Abstract
Lactic acid bacteria present in the reproductive secretions of cows with different clinical types were screened,identified,and analyzed for flora diversity to assess their microecological system in Southern Xinjiang.The cows were divided into two groups,healthy(n=3)and diseased(n=3)with endometritis.Samples of reproductive tract secretions were collected from each group.The flora present in the reproductive tract secretions of dairy cows were analyzed using methods such as isolation,purification,microscopic examination,16S rRNA sequencing,and high-throughput sequencing.The results showed that globular morphology and 17 strains of Gram-positive bacteria were observed during the preliminary is olation and microscopic examination after Gram staining.High-through-put sequencing yielded 652 901 valid sequences.After mass filtering and denoising,5 372 amplicon sequence variant(ASV)were ob-tained.The most significant variability was observed at the genus taxonomic level for Corynebacterium and at the lactic acid bacteria level for Streptococcus.Alpha diversity analysis revealed that the community richness and diversity of samples from the reproductive tract of healthy dairy cows were significantly higher than those from the diseased group.Beta diversity analysis also indicated that after hierarchical clustering,the group clustered the six samples into two groups.This suggests that the differences in colony similarity be-tween the groups were significant(P=0.05)while the intra-group differences were small.Beta diversity analysis also showed that the six samples were clustered into two groups by group,indicating small intra-group differences and large colony similarities between the groups.There were differences in the genus taxonomic level of the secretory flora of the reproductive tract of cows with different clinical types.The richness and diversity of the reproductive tract flora of healthy cows were significantly higher than those of the diseased cows.Further study is needed to explore the relationship between these differences and endometritis.关键词
乳酸菌/奶牛生殖道/子宫内膜炎/分离鉴定Key words
lactic acid bacteria/reproductive tract of dairy cows/endometritis/isolation and identification分类
农业科技引用本文复制引用
李仁平,陈荣,杨颖,李平,王梦瑶,石春华,佐丽卡尔爱·吐尔逊江,李涛..不同临床型奶牛生殖道乳酸菌的筛选鉴定及菌群多样性分析[J].塔里木大学学报,2024,36(2):19-29,11.基金项目
塔里木大学大学生创新创业训练计划项目(2022016) （2022016）
