妇儿健康导刊2024，Vol.3Issue(10)：188-194,7.
人乳头瘤病毒多重感染患者阴道菌群特征及功能分析
Characteristics and functional analysis of vaginal microbiota in patients with multiple human papilloma virus infection
摘要
Abstract
Objective To analyze the characteristics and function of vaginal microbiota in patients with multiple human papilloma virus(HPV)infection.Methods A total of 20 patients who underwent HPV typing detection in the Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine from June 2022 to January 2023 were selected,including 10 cases of single HPV infection and 10 cases of multiple HPV infection.The vaginal flora was detected by 16Sr DNA high-throughput sequencing,and the function of vaginal flora was analyzed by KEGG.Results The Shannon index and Simpson index of patients with single HPV infection were higher than those with multiple HPV infection,and the differences were statistically significant(P=0.032,0.027).The dominant bacteria in patients with single HPV infection were Lactobacillus,Gardnerella,Streptococcus,Atopobium,and Mycoplasma.The dominant vaginal bacteria in patients with multiple HPV infection were Gardnerella,Lactobacillus,Prevotella,Mycoplasma,and Megasphaera.Aquicella in patients with HPV single infection was higher than that in patients with multiple HPV infection(P=0.000).The Subdoligranulum and Truepera genera of patients with multiple HPV infection were higher than those with single HPV infection(P=0.002,0.000).The functions of the microbiota in HPV single infection mainly focused on protein folding,biosynthesis of other secondary metabolites,nucleotide metabolism,and protein translation.The functions of vaginal flora in patients with multiple HPV infection mainly focused on the biosynthesis and metabolism of glycans,metabolism of other amino acids,biodegradation and metabolism of xenoorganisms,amino acid metabolism,energy metabolism,cofactor and vitamin metabolism,etc.Conclusion The diversity of vaginal microbiota in patients with multiple HPV infection is decreased,and the function is more inclined to the metabolism of related amino acids and biological factors.关键词
人乳头瘤病毒/多重感染/阴道菌群/功能分析Key words
Human papilloma virus/Multiple infection/Vaginal microbiota/Functional analysis分类
医药卫生引用本文复制引用
刘蕊,魏洪,蒋立娜,徐庆胜,王硕石,王皞..人乳头瘤病毒多重感染患者阴道菌群特征及功能分析[J].妇儿健康导刊,2024,3(10):188-194,7.基金项目
国家自然科学基金(3226010016) （3226010016）
贵州省中医药管理局中医药、民族医药科学技术研究专项(202449782) （202449782）
贵州省卫生健康委科学技术基金(gzwkj2023-280) （gzwkj2023-280）
贵州省教育厅平台项目(黔教技[2022]019号) （黔教技[2022]019号）
国家级大学生创新训练项目(202310660028) （202310660028）
省级大学生创新训练项目(S202310660027 （）
202310660049). （）
