心脑血管病防治2024，Vol.24Issue(5)：32-36,5.DOI:10.3969/j.issn.1009-816x.2024.05.008
急性心肌梗死患者微小RNA-325-3p水平与病情严重程度及预后的关系
Relationship between microRNA-325-3p level,severity and prognosis in patients with acute myocardial infarction
摘要
Abstract
Objective To investigate the relationship between microRNA-325-3p(miR-325-3p)level and the severity as well as prognosis in patients with acute myocardial infarction(AMI).Methods From July 2018 to June 2020,90 AMI patients admitted to Huairou District Traditional Chinese Medicine Hospital of Beijing were selected.The expression level of miR-325-3p in patients'plasma was detected by real-time quantitative PCR(RT-qPCR).According to Gensini score,AMI patients were classified into the mild disease group(28 cases),moderate disease group(36 cases),and severe disease group(26 cases).After 2 years of follow-up,they were grouped into the poor prognosis group(48 cases)and good prognosis group(42 cases)based on different prognosis.Spearman method was applied to analyze the correlation between plasma miR-325-3p expression and Gensini score,creatine kinase isoenzymes(CK-MB),B-type natriureticpeptide(BNP),as well as cardiactroponin(cTnI)in AMI patients.Kaplan-Meier survival curve was applied to analyze the relationship between miR-325-3p expression and the prognosis of AMI patients.Influencing factors for the poor prognosis in AMI patients were analyzed by Cox regression.Results The expression levels of miR-325-3p and Gensini scores among mild,moderate and severe disease groups were statistically significant different(F=39.031,78.573;P<0.01).Compared with the mild and moderate disease groups,plasma miR-325-3p expression level in the severe disease group was significantly decreased;compared with the mild disease group,plasma miR-325-3p expression level in the moderate disease group was significantly decreased(P<0.05).Compared with the good prognosis group,the expression level of miR-325-3p in the poor prognosis group was significantly decreased(t=7.525,P<0.05).Spearman correlation analysis showed that plasma miR-325-3p expression level in AMI patients was negatively correlated with Gensini score,CK-MB,BNP and cTnI(r=-0.616,-0.608,-0.574,-0.642;P<0.01).Kaplan-Meier analysis showed that the incidence of poor prognosis in the miR-325-3p low expression group was obviously higher than that in the miR-325-3p high expression group(χ2=18.581,P<0.01).Multivariate Cox regression analysis showed that Killip grade(≥grade 3)and cTnI were risk factors for the poor prognosis of AMI patients,while miR-325-3p and LVEF were protective factors(HR=1.405,1.628,0.794,0.816;P<0.05).Conclusion The expression level of miR-325-3p is closely related to the severity and prognosis in AMI patients,thus can be a potential biomarker for predicting the poor prognosis of AMI patients.关键词
微小RNA-325-3p/急性心肌梗死/病情严重程度/预后Key words
MicroRNA-325-3p/Acute myocardial infarction/Disease severity/Prognosis引用本文复制引用
林丹凤,陈艳俏,牛卫洲..急性心肌梗死患者微小RNA-325-3p水平与病情严重程度及预后的关系[J].心脑血管病防治,2024,24(5):32-36,5.基金项目
北京市怀柔区卫生和计划生育委员会资助项目(2018-B-002) （2018-B-002）
