针刺研究2024，Vol.49Issue(6)：551-557,7.DOI:10.13702/j.1000-0607.20230820
艾灸"心俞""肺俞"对慢性心力衰竭大鼠心肌瞬时受体电位香草酸亚型1、降钙素基因相关肽表达的影响
Effect of moxibustion at"Xinshu"(BL15)and"Feishu"(BL13)on expression of TRPV1 and CGRP in the myocardial tissue of chronic heart failure rats
摘要
Abstract
Objective To observe the effect of moxibustion at"Xinshu"(BL15)and"Feishu"(BL13)on transient receptor potential vanilloid type 1(TRPV1),calcitonin gene-related peptide(CGRP),and serum interleukin-10(IL-10)in the myocardial tissue of rats with chronic heart failure(CHF),so as to explore its underlying mechanisms in improvement of CHF.Methods Male SD rats were randomly divided into the normal,model,moxibustion,capsaicin,moxibustion+capsaicin,and moxibustion+solvent groups,with 10 rats in each group.The CHF model was established by permanent ligation of the anterior descending branch of the left coronary artery.Mild moxibustion was applied to bilateral BL13 and BL15 for 30 min once daily for 4 weeks.Rats in the capsaicin group were smeared with capsaicin in the acupoint area once a day for 4 weeks.For rats of the moxibustion+capsaicin and moxibustion+solvent groups,capsaicin and solvent were applied to the acupoint area before moxibustion for 4 weeks,respectively.The ejection fraction(EF)and left ventricular fractional shortening rate(FS)were examined by echocardiography.HE staining was used to observe the myecardial morphological structure.The mRNA and protein expression levels of TRPV1,CGRP and galectin-3(Gal-3)in myocardial tissue were detected by real-time quantitative PCR and Western blot,respectively.The content of IL-10 in serum was detected by ELISA.Results After modeling,the pathological changes of myocardium(as cardiac muscle fiber disorder,inflammatory cell infiltration,etc.)were obvious,and the EF,FS,serum IL-10,protein and mRNA exspression of TRPV1 and CGRP were significantly decreased(P<0.01)in the model group compared with the normal group,while the protein and mRNA exspression of Gal-3 were significantly up-regulated(P<0.01).Following the interventions,the above-mentioned indexes were all reversed in moxibustion,capsaicin,and moxibustion+capsaicin groups(P<0.01),and the effect of moxibustion+capsaicin was the best(P<0.05,P<0.01).Conclusion Moxibustion can reduce myocardial injury and improve cardiac function in CHF rats,which may be related to its effects in up-regulating the expression of TRPV1 and CGRP,and down-regulating the expression of Gal-3 to alleviate myocardial fibrosis.关键词
慢性心力衰竭/心肌纤维化/艾灸/瞬时受体电位香草酸/降钙素基因相关肽Key words
Chronic heart failure/Myocardial fibrosis/Moxibustion/Transient receptor potential channel vanilloid/Calcitonin gene-related peptide引用本文复制引用
朱玲,高兵,宫甜甜,刘攀,李澜,夏冉,王茎..艾灸"心俞""肺俞"对慢性心力衰竭大鼠心肌瞬时受体电位香草酸亚型1、降钙素基因相关肽表达的影响[J].针刺研究,2024,49(6):551-557,7.基金项目
国家自然科学基金面上项目(No.81574084) （No.81574084）
新安医学与中医药现代化研究所"揭榜挂帅"项目(No.2023CXMMTCM022) （No.2023CXMMTCM022）
安徽省教育厅重点项目(No.KJ2021A0570、2023AH050796) （No.KJ2021A0570、2023AH050796）
安徽省高校科研项目重点项目(No.2023AH050796、2023AH050818) （No.2023AH050796、2023AH050818）
