中国农业大学学报2024，Vol.29Issue(8)：120-133,14.DOI:10.11841/j.issn.1007-4333.2024.08.10
发酵乳对肥胖小鼠肝脂代谢和肠道菌群的影响
Effects of fermented milk on liver lipid metabolism and intestinal flora in obese mice
摘要
Abstract
To analyze the effects of fermented milk on liver lipid metabolism and intestinal microbiota in obese mice,sixty C57BL/6 mice were randomly divided into five groups:control group(CON),model group(MD),Yishanfu group(YG),low concentration fermented milk intervention group(LFG),and high concentration fermented milk intervention group(HFG).After successful modeling of obesity,a 6-week gastric lavage was performed.At the end of the experiment,the weight and length of the mice were measured after fasting,the LEE'S index was calculated,the blood was collected for biochemical indicators and pathological changes in liver tissue were observed,and cecal contents were obtained from mice for gut microbiota sequencing.The results showed that:1)Compared with CON,the body weight,LEE'S index,ALT,and AST of MD mice were significantly increased(P＜0.01),indicating that the obesity model was successfully established.2)Compared with MD,the body weight,LEE'S index,ALT,and TC of the fermented milk intervention group mice were significantly reduced(P＜0.01),with AST and TG significantly reduced(P≤0.05).At the same time,the fatty degeneration of liver tissue in the fermented milk and Yishanfu group of mice was significantly improved,with almost no obvious fat vacuoles,indicating that fermented milk can inhibit the weight gain of obese mice,and improve their liver lipid metabolism function.3)Compared with MD,the diversity level of intestinal microbiota in the intervention group of mice showed a certain degree of recovery,especially in the Firmicutes and Bacteroidetes.It indicated that fermented milk had a certain effect on the restoration of intestinal microbiota diversity in mice.In summary,this study clarified that effects of fermented milk on liver lipid metabolism and intestinal flora in obese mice,providing a theoretical basis for the development of functional fermented milk.关键词
发酵乳/肥胖/肝脂代谢/肠道菌群Key words
fermented milk/obesity/liver lipid metabolism/intestinal microbiota分类
农业科技引用本文复制引用
李昊霞,张道亮,薛之霖,张天琳,冯士彬,赵畅,王希春,李玉..发酵乳对肥胖小鼠肝脂代谢和肠道菌群的影响[J].中国农业大学学报,2024,29(8):120-133,14.基金项目
优才计划学生发展支持项目(XSZZ2021003) （XSZZ2021003）
