gga-miR-17-5p在鸡骨骼肌和脂肪沉积相关组织及细胞中的表达差异及其关键靶基因的筛选OA北大核心CSTPCD

To investigate the role of gga-miR-17-5p in chicken fat deposition and muscle development,the study used dwarf strain S3(DW)and recessive white feathered chickens(RR)as test materials to analyze the expression differences of gga-miR-17-5p in bone and adipose tissues and cells at different periods of time,and to screen the key target genes by KEGG and protein interactions analysis.The results showed that:1)gga-miR-17-5p was expressed in abdominal fat,liver and leg muscle tissues.In abdominal fat,there was a variety difference in the expression of gga-miR-17-5p at 0 week(P＜0.05),and the two varieties were significantly higher in two breeds at week 0 than other weekly ages(P＜0.05).In liver,gga-miR-17-5p was expressed with breed differences at week 16(P＜0.05),and was significantly higher in S3 line at week 16 than other weekly ages(P＜0.05),and was significantly higher in cryptic white-feathered broilers at 16 weeks was significantly higher than at 0 and 8 weeks(P＜0.05).In the leg muscle,there was a breed difference in gga-miR-17-5p at 0 weeks(P＜0.05),and the S3 line was significantly higher than other weeks at 0 and 2 weeks(P＜0.05),The recessive white feathered chickens were significantly higher than other weeks of age at 0 weeks(P＜0.05).2)In adipocytes,the expression of gga-miR-17-5p in intramuscular adipocytes after 6 days of induction of differentiation was significantly higher than that in the proliferation phase and 1 day of differentiation.(P＜0.05)The lowest expression was found in abdominal adipocytes at 1 d of differentiation,which was significantly lower than that in the proliferative stage,at 4 d of differentiation,and at 6 d of differentiation(P＜0.05).3)In myoblasts,the expression of gga-miR-17-5p was elevated with the prolongation of the time of differentiation,and it was significantly higher in 6 d of differentiation than that in the proliferative stage(P＜0.05).4)KEGG and protein interactions analysis showed that PTEN,MAPK3,PIK3R1,BECN1 and PLCB4 were important target genes of gga-miR-17-5p.The RT-qPCR results showed that the expression of PTEN and PIK3R1 was significantly higher in the proliferative stage of myoblasts than that in the differentiation stage(P＜0.05).Taken together,the expression of gga-miR-17-5p exhibits significant species and tissue variability and miR-17-5p is likely to affect muscle development and fat deposition through PTEN,MAPK3,PIK3R1,BECN1 and PLCB4,of which PTEN and PIK3R1 are particularly critical.This study provides a solid theoretical foundation for the in-depth study of gga-miR-17-5p function and mechanism.