中国农业大学学报2024，Vol.29Issue(7)：34-46,13.DOI:10.11841/j.issn.1007-4333.2024.07.04
gga-miR-130b-3p对鸡MDCC-MSB1细胞中肿瘤相关基因表达的影响
Effects of gga-miR-130b-3p on the expression of tumour-related genes in chicken MDCC-MSB1 cells
摘要
Abstract
To identify tumour-associated genes regulated by gga-miR-130b-3p in MD lymphoma-transformed cells,in the study,the miRNA mimics was overexpressed in MDV-transformed chicken lymphoblastoid cell line MDCC-MSB1,and cells were collected and RNA extracted 48 h after transfection.The transcriptome data were obtained for the overexpression of gga-miR-130b-3p group and the negative control(NC)group using high-throughput sequencing.Screening these data for differentially expressed genes(DEGs),functional enrichment analysis of DEGs for Gene ontology(GO),signaling pathway analysis and Gene Set Enrichment Analysis(GSEA)were performed to screen the signaling pathways that may be involved in the changes in gene expression before and after overexpression of gga-miR-130b-3p and to analyze the differentially alternative splicing in the two groups.The results showed that:1)Compared to the NC group,a total of 117 DEGs were identified in the overexpression gga-miR-130b-3p group,of which 83 DEGs were significantly up-regulated and 34 DEGs were significantly down-regulated.Among them,MCM10,KCNA3,PTK2,FGL2,GPAM,BMP4,LOXL3,DDO and KRAS may affect MD tumor transformation.2)DEGs were annotated into 46 GO entries,of which 22 entries including immune system processes for biological processes,10 entries for molecular functions,and 14 entries for cellular components.3)DEGs in the mimics NC and mimics transfected groups as well as genes of small effect were enriched to seven pathways associated with tumourigenesis,which mainly exerted oncogenic effects through the thyroid hormone signaling pathway,choline metabolism and other pathways.4)The most differentially alternative splicing type after overexpression of gga-miR-130b-3p was exon skipped(ES),followed by intron retained(IR),and the least was mutually exclusive exon(MXE).Taken together,high expression of gga-miR-130b-3p can cause differential expression of some genes in MSB1 cells,and these DEGs inhibit tumour progression in MD through oncogenic signaling pathways.This study may provide a theoretical basis for analyzing the mechanism of gga-miR-130b-3p in MD tumour transformation process.关键词
gga-miR-130b-3p/MD肿瘤相关基因/转录组测序/生物信号通路Key words
gga-miR-130b-3p/Marek's disease/transcriptome sequencing/biosignaling pathway分类
农业科技引用本文复制引用
李家乐,金涛,朱国志,陈沈峻,杨林,刘心雨,李升和,赵春芳..gga-miR-130b-3p对鸡MDCC-MSB1细胞中肿瘤相关基因表达的影响[J].中国农业大学学报,2024,29(7):34-46,13.基金项目
国家自然科学基金项目(32002160,32172816) （32002160,32172816）
安徽省教育厅青年拔尖人才项目 （）
安徽省高校科研项目计划自然科学重点项目(2023AH051872) （2023AH051872）
安徽省重点研究与开发计划项目(202204c06020074) （202204c06020074）
安徽科技学院人才引进项目(DKYJ201901) （DKYJ201901）
安徽省研究生创新创业实践项目(2023cxcysj175) （2023cxcysj175）
大学生创新创业训练计划项目(202110879059,S202210879200,S202210879194,X202310879019) （202110879059,S202210879200,S202210879194,X202310879019）
