中国农业大学学报2024，Vol.29Issue(7)：47-57,11.DOI:10.11841/j.issn.1007-4333.2024.07.05
转录组测序筛选萨湖杂交羊肌肉和生长性能提升的关键基因
Transcriptome sequencing screens key genes for muscle and growth performance enhancement in Sahu crossbred sheep
摘要
Abstract
To analyze the key genes and regulatory pathways involved in the muscle and growth performance enhancement of Sahu crossbred sheep compared to Hu sheep,in the study,the growth data of body weight,body height,body length,chest circumference and cannon circumference of F,generation of Suffolk and Hu sheep hybrids(SH)and Hu sheep(H)at 2 days and 8 months of age were compared and analyzed.Three sheep of 2-day-old and 8-month-old were randomly selected from each group(12 sheep in total).RNA-seq was used to sequence the transcriptome of longissimus dorsi muscle,and bioinformatics analysis was performed on the sequencing data.Ten differentially expressed genes were randomly selected and their expression levels were verified by qRT-PCR.The results showed that:1)The body weight,chest circumference and cannon circumference of Sahu hybrid sheep at 2 days and 8 months were significantly higher than those of Hu sheep(P＜0.001).2)A total of 684 and 248 genes with significant differential expression were screened in 2-day-old and 8-month-old SH and H,respectively,including CDH1,GSTA1-1,GPX2,CDH17,MX2 and other genes related to skeletal muscle growth and development.3)GO and KEGG analysis showed that the differentially expressed genes were mainly enriched in the categories of cell differentiation,nucleosome organization and actin filament regulation controlling muscle growth and development in 2-day-old SH and H,while the differentially expressed genes were mainly enriched in the categories of cell-cell adhesion,glutathione metabolism and PPAR signaling pathway in 8-month-old SH and H.4)The results of qRT-PCR were consistent with the transcriptome sequencing.In summary,the growth performance of Sahu hybrid sheep is better than that of Hu sheep.CDH1,GSTA1-1 and GPX2 genes play important roles in muscle development of Sahu crossbred sheep through G protein-coupled receptors,cell-cell adhesion,and glutathione metabolic pathways.The study provides a reference for exploring the key genes and functional pathways that regulate the growth performance of hybrid sheep.关键词
萨湖杂交F1代/湖羊/转录组/生长性能/关键基因/代谢通路Key words
Sa-Hu hybrid F1/Hu sheep/transcriptome/growth performance/key genes/metabolic pathways分类
农业科技引用本文复制引用
楼梦雨,张思欢,郑琪,朱翠云,凌英会..转录组测序筛选萨湖杂交羊肌肉和生长性能提升的关键基因[J].中国农业大学学报,2024,29(7):47-57,11.基金项目
国家重点研发计划项目(2022YFD1300202) （2022YFD1300202）
国家现代肉羊产业技术体系项目(CARS-38,2021-2025) （CARS-38,2021-2025）
安徽省肉羊良种联合攻关项目(2021-2025) （2021-2025）
