肉桂精油对苹果腐烂病菌和番茄灰霉病菌抑制作用及机理的初步研究OA北大核心CSTPCD

In order to investigate the inhibitory effect of Cinnamon essential oil(CEO)on Valsa mali and Botrytis cinerea and investigate its inhibitory mechanism,the mycelial growth rate and fumigation methods were adopted to observe the colony diameters of Valsa mali and Botrytis cinerea in different mass concentrations of CEO and the inhibitory effects of CEO on Valsa mali and Botrytis cinerea in vivo were determined.The effects of CEO on the morphology,cell membrane permeability,superoxide dismutase(SOD)enzyme activity,malondialdehyde(MDA)content,soluble protein content and reducing sugar content of the mycelium of Valsa mali and Botrytis cinerea were investigated.The results showed that:1)When the mass concentration of CEO was 250 mg/L,the inhibition rates of Valsa mali and Botrytis cinerea measured by mycelial growth rate method reached 98.31％and 99.06％,respectively;When the mass concentration of CEO was 8 000 mg/L,the inhibition rates of Valsa mali and Botrytis cinerea measured by fumigation method were 50.57％and 58.79％,respectively.2)When the mass concentration of CEO was 5 000 mg/L,the in vivo inhibitory effects on Valsa mali and Botrytis cinerea was 54.30％and 64.10％,respectively.3)The mycelia of Valsa mali and Botrytis cinerea treated with CEO were dried out,collapsed with bifurcation on the surface,and the bacterial liquid conductivity increased,the bacterial SOD enzyme activity decreased,MDA content increased,soluble proteins and content of reducing sugar decreased.Ultimately disturbed the physiological and biochemical reactions of the bacterium and achieved the effect of bacterial inhibition.This study provided a scientific basis for the development of plant-derived pesticides for the control of Valsa mali and Botrytis cinerea.