金樱子滴眼液对糖尿病性白内障大鼠晶状体氧化应激及p38 MAPK信号通路的调节作用OA

Objective To study the regulatory effect of Rosa laevigata Michx.(RLM)eye drops on oxidative stress and p38 MAPK signal pathway in lens of diabetic cataract rats.Methods 35 male SD rats were randomly divided into the control group(CG),the diabetic cataract model group(DC),RLM low-dose group,RLM medium-dose group and RLM high-dose group,with 7 rats in each group.The diabetic cataract model was established by intraperitoneal injection of 65mg/kg streptozotocin.The RLM eye drop intervention group(IG)was treated with RLM eye drop at 0.01mg/ml,0.05 mg/ml and 0.1 mg/ml,4 times a day for 12 weeks.The weight of rats were measured weekly,and the opacity of the lens was observed by slit-lamp at the 6th and 12th week.After the intervention,the levels of malondialdehyde,superoxide dismutase and glutathione peroxidase in the lens tissues of rats were detected.The expression levels of apoptotic proteins Bcl-2 and Bax,as well as p38 MAPK,p-p38 MAPK,MKK6 and p-MKK6 signaling pathway proteins were detected by Western blot.Results There was no significant difference in body weight among all groups before administration(P＞0.05).During the intervention period,the body weight of rats in the model group was significantly lower than that in the control group(P＜0.05),and the body weight of rats in the intervention group was higher than that in the model group(P＜0.05).The opacity of lens in the model group was higher than that in the control group(P＜0.05),and the opacity of lens in the intervention group was lower than that in the model group after treatment(P＜0.05).MDA levels in the lens of rats in model group were higher than those in control group(P＜0.05),while SOD and GSH-Px levels were lower than those in control group(P＜0.05).After treatment,the MDA level in the intervention group was lower than that in the model group,and the SOD and GSH-Px levels were higher than that in the model group,with statistical significance(P＜0.05).The expression level of apoptosis protein Bcl-2 in lens of model group was lower than that of control group(P＜0.05),the expression level of Bax protein was higher than that of control group(P＜0.05),and the value of Bcl-2/Bax was lower than that of control group(P＜0.05).After treatment,the expression level of apoptosis protein Bcl-2 in the lens of the intervention group was higher than that of the model group(P＜0.05),the expression level of Bax was lower than that of the model group(P＜0.05),and the value of Bcl-2/Bax was higher than that of the model group(P＜0.05).The expression levels of P-MKK6 and P-P38 MAPK protein in lens of model group were higher than those of control group(P＜0.05).After treatment,the expression levels of P-MKK6 and P-P38 MAPK protein in the intervention group were lower than those in the model group(P＜0.05).Conclusion By inhibiting p38 MAPK signaling pathway,lens oxidative stress and apoptosis,the RLM eye drops may improve lens function and delay the progression of diabetic cataract in rats.