中国药物经济学2024，Vol.19Issue(5)：105-108,4.DOI:10.12010/j.issn.1673-5846.2024.05.019
不同产地木瓜饮片的高效液相色谱法指纹图谱
HPLC Fingerprint on Chaenomelis Fructus Pieces from Different Areas
摘要
Abstract
Objective To establish HPLC fingerprint of Chaenomelis Fructus pieces and control thequality.Methods The method was HPLC.Stationary phase was Kromasoil C18(250×4.6 mm,5 μm).Mobile phase consisted of acetonitrile-0.4%H3PO4 in a gradient mode.Flow rate was 1.0 ml/min.Detection wavelength was 210 nm,column temperature was 30℃.The HPLC chromatograms of 13 batches of Chaenomeles were compared and analyzed to evaluate the similarity and cluster analysis.Results Nine common peaks were identified in the HPLC fingerprint of Chaenomeles Fructus pieces.The similarities exceeds 0.90 except sample 9.The relative peak areas of the common chromatographic peaks of 13 papaya samples were analyzed by systematic cluster analysis.The 13 samples were divided into 3 categories.The results showed that S9 was far away from other samples.Conclusion This method was stable,accurate and reproducible,it can be used for the overall quality control of Chaenomelis Fructus pieces.关键词
木瓜饮片/高效液相色谱法指纹图谱/质量控制Key words
Chaenomelis Fructus pieces/HPLC fingerprint/Quality control分类
医药卫生引用本文复制引用
王云,齐红,李敏..不同产地木瓜饮片的高效液相色谱法指纹图谱[J].中国药物经济学,2024,19(5):105-108,4.基金项目
山东省中医药管理局2017-2018中医药科技发展项目(2017-313) （2017-313）
