中华骨质疏松和骨矿盐疾病杂志2024，Vol.17Issue(2)：123-130,8.DOI:10.3969/j.issn.1674-2591.2024.02.004
TGF-β1通过组蛋白修饰酶调控人骨髓间充质干细胞成骨分化的表观基因学研究
Epigenetic study of TGF-β1 regulation on osteogenic differentiation of human bone marrow mesenchymal stem cells through histone modifying enzymes
摘要
Abstract
Objective To investigate the relationship between transforming growth factor β1(TGF-β1)/Smad signaling pathway and histone methylase/demethylase during osteogenic differentiation of bone marrow mesenchy-mal stem cells(BMSCs).Methods BMSCs were subjected to osteogenic induction,then TGF-β1,SB431542 and TGF-β1+SB431542 were incubated in the osteogenic induction medium.The BMSCs were divided into four groups:BMSCs-Ctrl-3d group(group A),BMSCs-Ctrl-ost3d group(group B),BMSCs-TGF-β1-ost3d group(group C),BM-SCs-TGF-β1-SB1UM-ost3d group(group D).qRT-PCR was used to test the mRMA expression levels of histone lysine methyltransferase/demethylase.Results Compared with group A,mRNA expressions of KDM3B,KDM4A,KDM4B,KDM4C,KDM4D,KDM5A,KDM5B,KDM5C,KDM6A,KDM6B and EZH1 in group B were significantly increased(P＜0.05).Compared with group B,the expressions of KDM3B,KDM4A,KDM4C,KDM4D,KDM5C and EZH1 in group C were significantly decreased(P＜0.05).The mRNA expressions of KDM5B,KDM6A and KDM6B in group C were significantly increased(P＜0.05).The changes of mRNA expressions of KDM4B and KDM5A were not significant(P＞0.05).Compared group C and group D,the mRNA expressions of KDM3B,KDM6A and KDM6B were significant-ly different(P＜0.05).The changes of KDM4C,KDM4D,KDM5C and EZH1 were not significant(P＞0.05),and the mRNA expression change of KDM4A was significant but non-specific(P＜0.05).Conclusion Many kinds of his-tone lysine methyltransferases and demethylases were regulated by both TGF-β1/Smad and TGF-β1/non-Smad signaling pathway in BMSCs bone differentiation.关键词
骨髓间充质干细胞/成骨分化/组蛋白甲基化酶/TGF-β1/Smad信号通路Key words
bone marrow mesenchymal stem cells(BMSCs)/osteogenic differentiation/histone methylase/transforming growth factor β1(TGF-β1)/Smad signaling pathway分类
医药卫生引用本文复制引用
何建伟,晏春艳,杨宗树,高永昌,蒋雯,方钦正,戴晓琴,曹薇薇..TGF-β1通过组蛋白修饰酶调控人骨髓间充质干细胞成骨分化的表观基因学研究[J].中华骨质疏松和骨矿盐疾病杂志,2024,17(2):123-130,8.基金项目
石河子大学校级科研项目(ZZZC202142) （ZZZC202142）
石河子大学第一附属医院博士课题(BS202105) （BS202105）
兵团科技计划项目(2022ZD035) （2022ZD035）
