植物营养与肥料学报2024，Vol.30Issue(5)：996-1007,12.DOI:10.11674/zwyf.2023550
长期玉米秸秆还田对褐土细菌群落和酶活性的影响
Effects of long-term maize straw return on bacterial communities and enzyme activities in cinnamon soil
摘要
Abstract
[Objectives]We compared the beneficial effects of straw return modes on cinnamon soil chemical and biological fertility,to lay a basis for efficient culturing of fertile farmland.[Methods]The research was based on a 30-year long trial from 1992-2022,located in Shouyang,Shanxi Province.The experiment was composed of four maize straw return methods:not returned to field(CK),mulching on the field(SM),crushed and ploughed directly into field(SC),and returned to field after used as forage(CM).Soil nutrient content,enzyme activity,and bacterial community structure were comprehensively analyzed using high-throughput sequencing technology,FAPROTAX functional prediction,and ecological network methods.[Results]1)Long-term straw return(SM,SC and CM)significantly increased soil nutrient contents and extracellular enzyme activities.Compared with CK,the soil organic carbon,total N,total P,total K,alkali-dissolved N,available P,quickly available K contents were elevated in ranges of 17.0%-42.9%,3.0%-50.0%,2.3%-27.9%,4.4%-11.5%,11.2%-71.0%,14.1%-320.8%,17.1%-153.6%,soil glucosidase(β-GC),cellobiose hydrolase(CBH)and dehydrogenase(DHA)contents were elevated in ranges of 35.6%-190.7%,41.9%-58.6%,and 28.8%-773.8%,cumulative maize yields increased by 6.4%-23.0%,respectively.2)SM,SC and CM treatments increased soil bacterial Chao 1 index,Peilou's evenness index and Shannon's index,the relative abundance of Bacteroidetes,Proteobacteria,Firmicutes,and Actinobacteria,and decreased the relative abundance of Chloroflexi and Acidobacteria.Soil pH and organic carbon were important factors influencing bacterial community composition.3)The results of the network analysis showed that the indicator species of the straw return treatments belonged to different modules and were significantly correlated with soil nutrient content and enzyme activities.The Chryseolinea,enriched in the CM treatment,had formed specific functional microbial clusters through strong symbiotic relationships.4)Functional prediction based on FAPROTAX found that long-term straw return increased the potential of organic matter decomposition and nitrogen conversion of soil flora.[Conclusions]Long-term different straw return significantly enhanced soil fertility and maize yield,increased soil enzyme activity,significantly increased bacterial diversity,changed the structure and composition of bacterial communities,and improved the function of soil carbon and nitrogen cycling,thus promoting the formation of a suitable environment for crop and bacterial growth in cinnamon soil.关键词
秸秆还田方式/细菌群落/酶活性/土壤养分/高通量测序/网络分析Key words
straw return pattern/bacterial community/enzyme activity/soil nutrients/high throughput sequencing/network analysis引用本文复制引用
赵宇航,殷浩凯,解文艳,刘志平,周怀平,杨振兴..长期玉米秸秆还田对褐土细菌群落和酶活性的影响[J].植物营养与肥料学报,2024,30(5):996-1007,12.基金项目
山西省基础研究计划项目(202103021224124) （202103021224124）
国家重点研发计划项目(2021YFD1901101-4) （2021YFD1901101-4）
科技基础资源调查专项(2021FY100501) （2021FY100501）
山西省重点研发计划项目(202102140601010-4) （202102140601010-4）
山西省科技重大专项计划项目(202101140601026-4-5). （202101140601026-4-5）
