北京中医药大学学报2024，Vol.47Issue(6)：818-825,8.DOI:10.3969/j.issn.1006-2157.2024.06.013
麦粒灸对环磷酰胺诱导的免疫抑制小鼠Th1/Th2平衡及转录因子T-bet、GATA3的影响
Effects of grain-sized moxibustion on Th1/Th2 balance and transcription factors T-bet and GATA3 in immunosuppressed mice induced by cyclophosphamide
摘要
Abstract
Objective To observe the effects of grain-sized moxibustion on Th1 cell/Th2 cell(Th1/Th2)balance and transcription factors T-box transcription factor(T-bet)and GATA binding protein 3(GATA3)in immunosuppressive mice induced by chemotherapy.Methods According to the random number table method,32 SPF male CD-1(ICR)mice were randomly divided into the normal group,model group,levamisole hydrochloride group,and grain-sized moxibustion group,with eight mice per group.Except for the normal group,the immunosuppressive model was established by intraperitoneal injection of cyclophosphamide(80 mg/kg,once daily for three consecutive days).Mice in the levamisole hydrochloride group were given levamisole hydrochloride solution(10 mg/kg)by gavage.Mice in the grain-sized moxibustion group was given grain-sized moxibustion at"Guanyuan"(CV4),bilateral"Zusanli"(ST36),and bilateral"Sanyinjiao"(SP6),with three Zhuang at each acupoint for approximately 30 s each.The intervention was administered once daily for seven consecutive days.The general condition of mice was observed.The spleen mass and spleen index were detected.The pathological changes of spleen tissue were observed by HE staining.The protein and mRNA expressions of T-bet,GATA3,interferon-γ(IFN-γ),and interleukin(IL)-4 in spleen tissue of mice were detected by Western blotting and real-time PCR.The contents of IFN-γ,IL-2,and IL-4 in serum of mice were detected by enzyme-linked immunosorbent assay.Results Compared with the normal group,the mice in the model group were slow in response,unstable in gait;the spleen weight and spleen index were increased(P<0.05);the structure of spleen tissue was disordered,the mRNA and protein expressions of T-bet and IFN-γ in spleen tissue were decreased,and the mRNA and protein expressions of GATA3 and IL-4 were increased(P<0.05);the contents of IFN-γ and IL-2 in serum were decreased,and the content of IL-4 was increased(P<0.05).Compared with the model group,the general condition of mice in the levamisole hydrochloride group and the grain-sized moxibustion group was improved,the structure of spleen tissue was improved,the mRNA and protein expressions of T-bet and IFN-γ in spleen tissue were decreased,and the mRNA and protein expressions of GATA3 and IL-4 were increased(P<0.05);the contents of IFN-γ and IL-2 in serum were decreased,and the content of IL-4 was increased(P<0.05).Conclusion Grain-sized moxibustion can significantly improve the immunosuppressive symptoms induced by chemotherapy.The mechanism may be through regulating the expressions of transcription factors T-bet and GATA3,regulating Th1/Th2 balance,and thus restoring the immune balance.关键词
麦粒灸/环磷酰胺/免疫抑制/Th1细胞/Th2细胞平衡/T盒子转录因子/GATA结合蛋白3/小鼠Key words
grain-sized moxibustion/cyclophosphamide/immunosuppression/Th1 cell/Th2 cell balance/T-box transcription factor/GATA binding protein 3/mice分类
医药卫生引用本文复制引用
朱涛,王祯芝,任佳,程艳婷,高珍,冀雨芳,苗晋玲,冀来喜..麦粒灸对环磷酰胺诱导的免疫抑制小鼠Th1/Th2平衡及转录因子T-bet、GATA3的影响[J].北京中医药大学学报,2024,47(6):818-825,8.基金项目
国家自然科学基金项目(No.82074549) （No.82074549）
山西省中医药管理局科研项目(No.2022ZYYZ075,No.2023ZYYA105) （No.2022ZYYZ075,No.2023ZYYA105）
山西中医药大学 2023年度科技创新能力培育计划项目(No.2023PY-YS-15,No.2023PY-YS-04) National Natural Science Foundation of China(No.82074549) （No.2023PY-YS-15,No.2023PY-YS-04）
