北京中医药大学学报2024，Vol.47Issue(6)：853-862,10.DOI:10.3969/j.issn.1006-2157.2024.06.017
防风均一多糖SP800301的化学结构研究
Chemical structure of a polysaccharide SP800301 from Saposhnikoviae Radix
摘要
Abstract
Objective To perform structural analysis on the homogeneous polysaccharide SP800301 isolated from Saposhnikoviae Radix to determine its chemical structure.Methods Polysaccharides were separated and purified from Saposhnikoviae Radix using column chromatography,such as DEAE-cellulose and Sephadex G-75.The molecular weight distribution,monosaccharide composition,oligosaccharide fragments,sugar residue types,and glycosidic bond connection of the isolated homogeneous polysaccharide SP800301 from Saposhnikoviae Radix were analyzed using electrospray ionization multistage mass spectrometry,gas chromatography-mass spectrometry,and nuclear magnetic resonance to determine its structure.The appearance characteristics of the Saposhnikoviae Radix polysaccharide were characterized using electron and atomic force microscopy.Results Polysaccharide SP800301 extracted from Saposhnikoviae Radix had good homogeneity with a molecular weight of 9.1×104 g/mol.The uronic acid content was 52.72%.The monosaccharide group comprised rhamnose,galacturonic acid,glucose,galactose,and arabinose,with a molar ratio of 4.3:58.1:25.4:5.0:7.3.The polysaccharide was mainly composed of polygalacturonic acid as the primary chain.The branched chain was comprised of rhamnose,galacturonic acid,galactose,glucose,and arabinose,with arabinose at the end of the branched chain and part of the galacturonic acid in the sugar chain forming a methyl ester.Conclusion This study clarified the chemical structure of the homogeneous polysaccharide SP800301 from Saposhnikoviae Radix,enriched the chemical composition of Saposhnikoviae Radix polysaccharides,laid the foundation for further research on the immune regulatory mechanism of Saposhnikoviae Radix polysaccharides,and provided a scientific basis for clinically using Saposhnikoviae Radix.关键词
防风/多糖/化学结构/半乳糖醛酸/阿拉伯糖/物质基础Key words
Saposhnikoviae Radix/polysaccharides/chemical structure/galacturonic acid/arabinose/material basis分类
医药卫生引用本文复制引用
王欣钰,范海涛,何新阳,孙萌,姜艳艳,刘斌..防风均一多糖SP800301的化学结构研究[J].北京中医药大学学报,2024,47(6):853-862,10.基金项目
国家自然科学基金项目(No.82074283) （No.82074283）
中央高校基本科研业务费专项资金资助(No.2023-JYB-KYPT-15) National Natural Science Foundation of China(No.82074283) （No.2023-JYB-KYPT-15）
