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基于UPLC-MS/MS对辣椒红类胡萝卜素代谢组的定量分析

李远桥 胡杰 吴雯 耿放 王金秋 吴笛

成都大学学报(自然科学版)2024,Vol.43Issue(2):143-149,165,8.
成都大学学报(自然科学版)2024,Vol.43Issue(2):143-149,165,8.DOI:10.3969/j.issn.1004-5422.2024.02.005

基于UPLC-MS/MS对辣椒红类胡萝卜素代谢组的定量分析

Quantitative Analysis of Paprika Oleoresin Carotenoid Metabolome Based on UPLC-MS/MS

李远桥 1胡杰 2吴雯 3耿放 1王金秋 1吴笛4

作者信息

  • 1. 成都大学食品与生物工程学院,四川 成都 610106
  • 2. 成都大学机械工程学院,四川 成都 610106
  • 3. 成都大学药学院,四川成都 610106
  • 4. 成都大学食品与生物工程学院,四川 成都 610106||成都大学机械工程学院,四川 成都 610106||成都大学药学院,四川成都 610106
  • 折叠

摘要

Abstract

To obtain a comprehensive understanding of the composition and content of carotenoids in pap-rika oleoresin,the researchers performed qualitative and quantitative analysis of them,before and after sa-ponification treatment by using ultra-high liquid chromatography coupled with tandem mass spectrometry(UPLC-MS/MS)based technique.The results showed that 41 carotenoids were detected in unsaponified paprika oleoresin,with 52.92%carotene content and 47.08%lutein content.In the saponified paprika o-leoresin,18 kinds of carotenoids were detected,with 43.14%carotene content and 56.86%lutein con-tent.The carotenoids with higher content in paprika oleoresin before and after saponification were all(E/Z)-phytoene,β-carotene,capsanthin,zeaxanthin and β-cryptoxanthin.These results indicated that paprika oleo-resin is rich in carotenoids and this research can provide more information on the composition and content of its carotenoids and some theoretical basis for the utilization and exploitation of paprika oleoresin.

关键词

辣椒红/类胡萝卜素/UPLC-MS/MS/皂化

Key words

paprika oleoresin/carotenoid/UPLC-MS/MS/saponification

分类

轻工纺织

引用本文复制引用

李远桥,胡杰,吴雯,耿放,王金秋,吴笛..基于UPLC-MS/MS对辣椒红类胡萝卜素代谢组的定量分析[J].成都大学学报(自然科学版),2024,43(2):143-149,165,8.

基金项目

四川省自然科学基金(2023NSFC0183) (2023NSFC0183)

成都大学学报(自然科学版)

1004-5422

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