福建农业科技2024，Vol.55Issue(4)：39-45,7.DOI:10.13651/j.cnki.fjnykj.2024.04.007
5种消毒剂对微生物实验室环境分离菌的消毒效果验证及应用
Verification and Application of Disinfection Effect of Five Disinfectants on the Environmental Isolated Bacteria from Microbiology Laboratories
摘要
Abstract
The study the germicidal efficacy of five chemical disinfectants on the environmental isolated bacteria from the microbiology laboratory could provide a basis for the rational and effective use of disinfectants in the microbiology laboratories.The suspension quantitative germicidal test was used to observe the germicidal efficacy of 75%ethanol,70%isopropanol,2 g·L-1 benzalkonium bromide,400 mg·L-1 84-aerosol and hydrogen peroxide silver ion disinfectant(4.5%H2O2,80 mg·L-1 Ag+)on the environmental isolated bacteria from microbiology laboratory.The results showed that the killing logarithm values of bacterial propagules exposed to 75%ethanol,70%isopropanol,2 g·L-1 benzalkonium bromide and 400 mg·L-1 84-aerosol for 2 min were all greater than 5.00,which met the requirements of qualified disinfection.When the hydrogen peroxide silver ion disinfectant(4.5%H2O2,80 mg·L-1 Ag+)was used for 60 min,the killing log values of fungi were greater than 4.00,and the killing log values of Bacillus megatherium was greater than 5.74,which met the requirements of qualified disinfection,while the killing log values of Bacillus subtilis was 2.36,which did not meet the requirements of qualified disinfection.In summary,the bacteria isolated from the environment could more truly reflect the microbial contamination in the laboratory.It was suggested that the laboratory should verify the efficacy of disinfectants based on the environmental monitoring data.According to the results of efficacy verification,at least two or more disinfectants should be reasonably screened and used in rotation.关键词
环境分离菌/悬液定量杀菌试验法/消毒剂/效果Key words
Environmental isolated bacteria/Suspension quantitative germicidal test/Disinfectant/Efficiency分类
化学化工引用本文复制引用
林梦丹,詹清,周阿容,揭小玲,郑孝贤,王海龙,方东升..5种消毒剂对微生物实验室环境分离菌的消毒效果验证及应用[J].福建农业科技,2024,55(4):39-45,7.基金项目
福建省科技计划省属公益类项目(2021R1005005). （2021R1005005）
