东华大学学报（英文版）2024，Vol.41Issue(3)：263-274,12.DOI:10.19884/j.1672-5220.202309006
不同肝细胞配体修饰的壳聚糖作为肝组织再生支架材料的比较研究:制备、表征和评价
Comparative Study on Chitosan Modified with Different Hepatocyte Ligands as Scaffold Material for Liver Tissue Regeneration:Preparation,Characterization and Evaluation
摘要
Abstract
To achieve the ideal scaffolds for liver tissue regeneration,chitosan(CS)was modified with lactobionic acid(LA)or/and glycyrrhetinic acid(GA)to obtain LA-modified CS(LC),GA-modified CS(GC)and GA/LA-modified CS(GLC),and the composite nanofibrous scaffolds composed of silk fibroin(SF)and the above modified CS were fabricated by green electrospinning.Fourier transform infrared(FTIR)spectroscopy,nuclear magnetic resonance(NMR)spectroscopy and X-ray diffraction(XRD)patterns were used to characterize the chemical components and structures.The water contact angle was measured to evaluate the hydrophilicity,and thermal gravimetric analysis(TGA)was carried out to obtain the thermal properties.These scaffolds were hydrophilic,and their hydrophilicity and thermal stability decreased with the increase of the modified CS content,while their crystallinity increased.The scaffolds showed good performance in promoting the proliferation of the human hepatoma cell line(HepG2 cell)as well as their secretion of both albumin and urea.Furthermore,the scaffolds with LC had a better performance of hepatocellular compatibility than those with GC or GLC.关键词
甘草次酸(GA)/半乳糖/壳聚糖(CS)/丝素蛋白(SF)/肝组织工程Key words
glycyrrhetinic acid(GA)/galactose/chitosan(CS)/silk fibroin(SF)/liver tissue engineering分类
医药卫生引用本文复制引用
王哲,余凡,姜玉新,侯玉婷,帅兰,邓敏,王红声..不同肝细胞配体修饰的壳聚糖作为肝组织再生支架材料的比较研究:制备、表征和评价[J].东华大学学报（英文版）,2024,41(3):263-274,12.基金项目
Science & Technology Commission of Shanghai Municipality(No.20DZ2254900) （No.20DZ2254900）
Municipal Public Welfare Research Project from Jiaxing,Zhejiang Province(No.2022AY10001) （No.2022AY10001）
Open Project Program of Jiaxing Key Laboratory of Virus-Related Infectious Diseases. （）
