工业微生物2024，Vol.54Issue(3)：22-24,3.DOI:10.3969/j.issn.1001-6678.2024.03.008
抑制幽门螺杆菌乳酸菌的筛选及抑菌物质的特性研究
Screening of Lactic Acid Bacteria Inhibiting Helicobacter Pylori and Study on the Characteristics of Antibacterial Substances
摘要
Abstract
In order to obtain lactic acid bacteria that can inhibit Helicobacter pylori,this study has screened 10 strains of lactic acid bacteria from pickled vegetables and conducted experiments on inhibition of Helicobacter pylori using the Oxford Cup method on these 10 strains.The results show that three of these 10 strains,PW-2,PW-5,and PW-7,have antibacterial activity against Helicobacter pylori.The PW-2 strain has the largest antibacterial diameter.This strain has been identified as Lactobacillus plantarum through morphological characteristics and 16S rDNA gene sequence alignment analysis.Further research has been conducted on the antibacterial active substances of this strain,and after excluding lactic acid and H2O2,it still shows antibacterial activity.Then,the subsequent protease sensitivity test shows that the antibacterial substances in the fermentation broth of the strain are proteins or peptides.In addition,the antibacterial substances of strain PW-2 exhibit strong antibacterial activity under acidic conditions and have the certain temperature stability.关键词
幽门螺杆菌/乳酸菌/抑菌活性Key words
Helicobacter pylori/Lactic acid bacteria/bacteriostatic activity引用本文复制引用
武航婕,武秀秀,屠晨坤..抑制幽门螺杆菌乳酸菌的筛选及抑菌物质的特性研究[J].工业微生物,2024,54(3):22-24,3.基金项目
南京医科大学康达学院2021年度科研发展基金课题(KD2021KYJJZD023) （KD2021KYJJZD023）
南京医科大学康达学院第一期"科研人才培养计划"(KD2021KYRC021) （KD2021KYRC021）
南京医科大学康达学院第一期"科研创新团队项目"(KD2022KYCXTD003). （KD2022KYCXTD003）
