工业微生物2024，Vol.54Issue(3)：61-64,4.DOI:10.3969/j.issn.1001-6678.2024.03.020
高效液相色谱法测定疫苗菌体破碎液中D-核糖的技术研究
Determination of D-ribose in the Bbroken Liquid of Vaccines by High Performance Liquid Chromatography
摘要
Abstract
A high performance liquid chromatography method for quantitatively determining the D-ribose with refractive index detector(RID)is established.The measurement conditions for this method are as follows:the Agilent ZORBAX NH2 column,0.005 mol/L of diluted sulfuric acid solution as the mobile phase,50℃ column temperature and the 20 μL injection volume.The retention time of D-ribose is 3.684 min.The linear regression equation for obtaining D-ribose is y=194.037 72x+10.834 09,and the correlation is 0.999 99,in which the y means the mass concentration of D-ribose and the x means the corresponding peak area.This method exhibits good precision,stability,and reproducibility.The recovery of D-ribose ranges from 90.2％to 102％.This improved method has high sensitivity and can achieve effective separation of target peak and impurity peak.Therefore,it can be widely used for measuring the D-ribose levels in a variety of the broken liquid of vaccines.关键词
D-核糖/示差检测器/疫苗菌体破碎液Key words
D-ribose/refractive index detector(RID)/broken liquid of vaccines引用本文复制引用
黄婷,徐晓萍,彭雪琦..高效液相色谱法测定疫苗菌体破碎液中D-核糖的技术研究[J].工业微生物,2024,54(3):61-64,4.基金项目
江苏省生产力促进中心青年人才基金项目"HPV(人乳头瘤病毒)疫苗原液中糖类杂质成分测定技术研究及方法开发"(编号:D2023003). （人乳头瘤病毒）
