工业微生物2024，Vol.54Issue(3)：104-110,7.DOI:10.3969/j.issn.1001-6678.2024.03.030
基于紫胶红素的靶标有色化免疫层析试纸条联检大肠杆菌O157∶H7和沙门氏菌
Immunochromatographic Test Strips for Simultaneous Detection of Escherichia Coli O157∶H7 and Salmonella Based on Shellac Red Conjugate
摘要
Abstract
In order to get rid of the need for paired antibodies in immunochromatographic test strips,this paper designed a new type of target-colored immunochromatographic test strips.The immunochromatographic strips were"visualized"by the method of mordanting and dyeing with shellac red pigment and potassium aluminum sulfate dodecahydrate.The results showed that the limits of detection for Escherichia coli O157∶H7 and Salmonella were 106 CFU/mL,there was no cross-reactivity with common foodborne pathogens,and the two were detectable within 9 hours of enrichment in chicken breast,egg,and milk food matrices.This paper successfully establish a simple,low-cost,and more easily localized rapid detection immunochromatographic test strip,and provided a new approach for the design of subsequent immunochromatographic test strips.关键词
大肠杆菌O157∶H7/沙门氏菌/紫胶红素/靶标有色化/免疫层析试纸条Key words
Escherichia coli O157∶H7/Salmonella/Shellac red/Target Coloring/Immunochromatographic test strips引用本文复制引用
刘坤,王英林,黄志强,刘箐..基于紫胶红素的靶标有色化免疫层析试纸条联检大肠杆菌O157∶H7和沙门氏菌[J].工业微生物,2024,54(3):104-110,7.基金项目
国家重点研发计划高信噪比功能集成探针创制技术研究(2023YFF1105002). （2023YFF1105002）
