高校化学工程学报2024，Vol.38Issue(3)：449-458,10.DOI:10.3969/j.issn.1003-9015.2024.00.006
米根霉全细胞脂肪酶在化学-酶法环氧化反应体系中的稳定性
Stability of Rhizopus oryzae whole-cell lipase in a chemo-enzymatic epoxidation reaction
许丽莉 1赵晴晴 2李青云 2唐爱星 2刘幽燕2
作者信息
- 1. 广西大学 化学化工学院,广西 南宁 530004||北部湾大学 海洋学院,广西 钦州 535011
- 2. 广西大学 化学化工学院,广西 南宁 530004
- 折叠
摘要
Abstract
Effects of trisodium citrate dosage,glutaraldehyde cross-linking of cells,H2O2 dosage and recycling methods on catalyst stability were examined using α-pinene as the model substrate,in order to enhance the stability of Rhizopus oryzae CGMCC 3.5040 whole-cell lipase in a chemo-enzymatic epoxidation reaction system.The results indicate that peroxyacid could affect enzyme stability.Addition of 3.5 mmol trisodium citrate could form a hypertonic solution with a 30%H2O2 solution,which prevented cell swelling and neutralized excess peroxyacid,thereby improved reaction selectivity and cell recycling stability.The thermal stability,storage stability,and recycling stability of the whole-cell catalyst were significantly enhanced after glutaraldehyde cross-linking.The optimal H2O2 dosage for the α-pinene epoxidation was 5 mmol.Recycling could be achieved by directly separating the organic phase and adding fresh organic phase.After optimizing the recycling method,the catalyst retains a conversion rate of 77.3%even after the 7th usage.关键词
米根霉/全细胞脂肪酶/化学-酶法环氧化/过氧有机酸/稳定性Key words
Rhizopus oryzae/whole-cell lipase/chemo-enzymatic epoxidation/peroxyacid/stability分类
生物科学引用本文复制引用
许丽莉,赵晴晴,李青云,唐爱星,刘幽燕..米根霉全细胞脂肪酶在化学-酶法环氧化反应体系中的稳定性[J].高校化学工程学报,2024,38(3):449-458,10.
