广西科学2024，Vol.31Issue(2)：269-278,10.DOI:10.13656/j.cnki.gxkx.20230712.001
广西地区中华圆田螺不同地理种群遗传多样性分析
Genetic Diversity Analysis of Different Geographical Populations of Cipangopaludina cathayensis in Guangxi Region
摘要
Abstract
In order to explore the genetic diversity characteristics of Cipangopaludina cathayensis population in Guangxi,the Pearl River Basin,China,12 pairs of microsatellite primers were used to analyze the genetic diversity and genetic differentiation of 10 geographical populations of C.cathayensis in Guangxi.The results showed that a total of 140 alleles were detected at 12 pairs of microsatellite loci,and the Polymorphic Infor-mation Content(PIC)was in the range of 0.373-0.923,of which 9 were highly polymorphic loci and 3 were moderately polymorphic loci.The Shannon information index(I)of the 10 geographical populations was 0.415-1.226,the observed heterozygosity(Ho)was 0.231-0.540 and the expected heterozygosity(He)of 0.239-0.608,which indicated that these populations had high genetic diversity.The results of Analysis of Molecular Variance(AMOVA)showed that the genetic variation within populations accounted for 75%,and the genetic variation among populations accounted for 25%.The genetic differentiation index(Fst)was 0.248,and the genetic differentiation among populations was higher.The gene flow(Nm)was 0.391-21.807,and the gene exchange was frequent among the remaining 8 populations(Nm＞1.000)except for the Hechi Du'an(HCDA)and Baise Napo(BSNP)population.The analysis of population structure showed that the genetic distance between BSNP population and Yulin Luchuan(YLLC)population was relatively far(D=0.047),and the degree of genetic differentiation was high(Fst=0.277).The results of cluster analysis and Principal Coordinate Analysis(PCoA)showed that the HCDA and BSNP population were in a separate branch,indicating that this two populations was more distantly related to other populations.Mantel analysis found that geographical distance was not the main factor affecting the genetic distance among populations.In summary,the germplasm resources of C.cathayensis in Guangxi are good,and the HCDA population and BSNP populations still maintain good wild resources,which have potential development and utilization value.关键词
中华圆田螺/广西地区/微卫星/遗传多样性/遗传分化Key words
Cipangopaludina cathayensis/Guangxi region/microsatellite/genetic diversity/genetic differenti-ation分类
农业科技引用本文复制引用
周康奇,杜雪松,何苹萍,韦嫔媛,潘贤辉,李育森,林勇,彭金霞,陈秀荔,覃俊奇,陈忠,黄姻,张彩群..广西地区中华圆田螺不同地理种群遗传多样性分析[J].广西科学,2024,31(2):269-278,10.基金项目
国家重点研发计划项目(2022YFD2400704),广西科技基地与人才项目(AD21220010),广西自然科学基金项目(2020GXNSFBA297067),广西创新驱动发展专项资金项目(AA17204080-5)和国家现代农业产业技术体系广西特色淡水鱼产业创新团队建设项目(nycytxgxcxtd-2021-08)资助. （2022YFD2400704）
