福建农业科技2024，Vol.55Issue(4)：6-16,11.DOI:10.13651/j.cnki.fjnykj.2024.04.002
甜椒幼苗低温胁迫的生理响应与转录组分析
Physiological Response and Transcriptome Analysis of Sweet Pepper Seedlings Under Low Temperature Stress
摘要
Abstract
In order to clarify the molecular mechanism of sweet pepper in response to low temperature stress,the MDA content,SOD activity of sweet pepper seedlings and the relative parameters of photosynthesis under the low temperature stress were determined,and the transcriptome was sequenced and analyzed.The results showed that the content of MDA in sweet pepper increased significantly,the activity of SOD decreased significantly,and the photosynthetic relative parameters decreased significantly under the low temperature stress for 3 days.Compared with those before the treatment of low temperature,the transcriptome sequencing analysis showed that the differentially expressed genes were mainly involved in the enzyme system,photosynthesis,signal transduction and related transcription factors.Among them,the expression of SOD-related gene CaSOD was up-regulated,and the expression of photosynthesis key genes CaCP4 and CaHCR was significantly down-regulated.The results laid a foundation for the further analysis of the molecular mechanism of sweet pepper seedlings in response to the low temperature stress and the anti-low temperature breeding of sweet pepper.关键词
甜椒/低温胁迫/转录组/差异表达基因Key words
Sweet pepper/Low temperature stress/Transcriptome/Differentially expressed genes分类
农业科技引用本文复制引用
马馨怡,林艺辉,余松金,倪辉,林蔚,韦幂,陈汉鑫..甜椒幼苗低温胁迫的生理响应与转录组分析[J].福建农业科技,2024,55(4):6-16,11.基金项目
福建省科技计划引导性项目(2023N0054). （2023N0054）
